Different sensitivity in expression of transcription factor mRNAs in congenic mice following exposure to low-level toluene

Abstract

To investigate the relationship between sensitivity to toluene exposure and genetic background, male congenic mice, C57BL/10 (H-2^b) and B10.BR/Sg (H-2^k) were exposed to 0, 5, and 50 ppm toluene for 6 h per day, 5 days per week for 6 weeks. Groups of mice were injected with ovalbumin (OVA) intraperitoneally before starting exposure schedule and these mice were then challenged with aerosolized OVA as a booster. Following 24 h of the last exposure, the spleens were collected. We examined spleen cell proliferation using DNA synthesis and T-helper 1/2-related transcription factor genes in spleen of two congenic mice using real-time reverse transcriptase-polymerase chain reaction. Although lipopolysaccharide-induced (LPS-induced) cell proliferation was significantly increased in 50 ppm toluene-exposed, nonimmunized B10.BR mice but not in C57BL/10 mice, exposure to 50 ppm toluene significantly decreased LPS-induced cell proliferation in immunized B10.BR mice. The expression of transcription factor forkhead box P3, signal transducers and activators of transcription (STAT)5 and STAT6 mRNAs was significantly increased in spleen from 5 ppm toluene-exposed, OVA-immunized B10.BR mice, but not in those of C57BL/10 mice. Although there may be other differences unrelated to H-2 locus between the congenic mice of the same background, the findings of the present study strongly suggest a possible role of H-2 locus in the toluene-induced immune disturbance.

Keywords::

• Toluene
• cell proliferation
• spleen
• congenic mice
• transcription factor

Acknowledgements

The authors thank Drs. T. Ishidao, Y. Fueta, and H. Hori (University of Occupational and Environmental Health, Japan) for their helpful discussions. We also thank Mrs. S. Murakami, and K. Ohnishi (National Institute for Environmental Studies) for their excellent technical assistance.

Declaration of interest

This research was partly supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, Japan (#18390189, #21390198 to H. F).