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Inhalation Toxicology
International Forum for Respiratory Research
Volume 15, 2003 - Issue 1
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Research Article

Exposure of Human Lung Cells to Inhalable Substances: A Novel Test Strategy Involving Clean Air Exposure Periods Using Whole Diluted Cigarette Mainstream Smoke

Detlef Ritter Department of In Vitro Toxicology, Fraunhofer Institute of Toxicology and Aerosol Research, Hannover, Germany
,
Jan W. Knebel Department of In Vitro Toxicology, Fraunhofer Institute of Toxicology and Aerosol Research, Hannover, Germany
&
Michaela Aufderheide Department of In Vitro Toxicology, Fraunhofer Institute of Toxicology and Aerosol Research, Hannover, Germany
Pages 67-84 | Published online: 01 Oct 2008

  • Adams, L., Lee, C., Rawbone, R., and Guz, A. 1983. Patterns of somking: Measurement and variability in asymptomatic somkers. Clin. Sci. (Lond.) 65(4):383-392.
  • Anand, C. V., Anand, U., and Agarwal, R. 1996. Anti-oxidant enzymes, gamma-glutamyl transpeptidase and lipid peroxidation in kidney of rats exposed to cigarette smoke. Indian J. Exp. Biol. 34(5):486-488.
  • Aufderheide, M., and Mohr, U. 1999. CULTEX--A new system and technique for the cultivation and exposure of cells at the air/liquid interface. Exp. Toxicol. Pathol. 51(6):489-490.
  • Aufderheide, M., Ritter, D., Knebel, J. W., and Scherer, G. 2001. A method for in vitro analysis of the biological activity of complex mixtures such as sidestream cigarette smoke. Exp. Toxicol. Pathol. 53(2):141-152.
  • Baker, M. A., Cerniglia, G. J., and Zaman, A. 1990. Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal. Biochem. 190(2):360-365.
  • Bayram, H., Devalia, J. L., Sapsford, R. J., Ohtoshi, T., Miyabara, Y., Sagai, M., and Davies, R. J. 1998. The effect of diesel exhaust particles on cell function and release of inflammatory mediators from human bronchial epithelial cells in vitro. Am. J. Respir. Cell Mol. Biol. 18(3):441-448.
  • Boland, S., Bonvallot, V., Fournier, T., Baeza-Squiban, A., Aubier, M., and Marano, F. 2000. Mechanisms of GM-CSF increase by diesel exhaust particles in human airway epithelial cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 278(1):L25-L32.
  • Bombick, D. W., Bombick, B. R., Ayres, P. H., Putnam, K., Avalos, J., Borgerding, M. F., and Doolittle, D. J. 1997. Evaluation of the genotoxic and cytotoxic potential of mainstream whole smoke and smoke condensate from a cigarette containing a novel carbon filter. Fundam. Appl. Toxicol. 39(1):11-17.
  • Bombick, B. R., Murli, H., Avalos, J. T., Bombick, D. W., Morgan, W. T., Putnam, K. P., and Doolittle, D. J. 1998. Chemical and biological studies of a new cigarette that primarily heats tobacco. Part 2. In vitro toxicology of mainstream smoke condensate. Food Chem. Toxicol. 36(3):183-190.
  • Cantin, A. M., North, S. L., Hubbard, R. C., and Crystal, R. G. 1987. Normal alveolar epithelial lining fluid contains high levels of glutathione. J. Appl. Physiol. 63(1):152-157.
  • Cardile, V., Jiang, X., Russo, A., Casella, F., Renis, M., and Bindoni, M. 1995. Effects of ozone on some biological activities of cells in vitro. Cell Biol. Toxicol. 11(1):11-21.
  • Carmines, E. L. 2002. Evaluation of the potential effects of ingredients added to cigarettes Part 1: Cigarette design, testing approach, and review of results. Food Chem. Toxicol. 40(1):77-91.
  • Channarayappa Nath, J., and Ong, T. 1992. Clastogenic and aneuploidogenic effects of cigarette smoke condensate, mitomycin C and vincristine sulfate. Mutagenesis 7(6):457-460.
  • Cotgreave, I. A., Johansson, U., Moldeus, P., and Brattsand, R. 1987. The effect of acute cigarette smoke inhalation on pulmonary and systemic cysteine and glutathione redox states in the rat. Toxicology 45(2):203-212.
  • Don Porto Carero, A., Hoet, P. H., Verschaeve, L., Schoeters, G., and Nemery, B. 2001. Genotoxic effects of carbon black particles, diesel exhaust particles, and urban air particulates and their extracts on a human alveolar epithelial cell line (A549) and a human monocytic cell line (THP-1). Environ. Mol. Mutagen. 37(2):155-163.
  • Dumler, K., Hanley, Q. S., Baker, C., Luchtel, D. L., Altman, L. C., and Koenig, J. Q. 1994. The effects of ozone exposure on lactate dehydrogenase release from human and primate respiratory epithelial cells. Toxicol. Lett. 70(2):203-209.
  • Dunnill, M. S. 1962. Postnatal growth of the lung. Thorax 17:329.
  • Eke, B. C., Vural, N., and Iscan, M. 1996. Combined effects of ethanol and cigarette smoke on hepatic and pulmonary xenobiotic metabolizing enzymes in rats. Chem. Biol. Interact. 102(3):l55-l67.
  • Emura, M., Riebe, M., Ochiai, A., Aufderheide, M., Germann, P., and Mohr, U. 1990. New functional cell-culture approach to pulmonary carcinogenesis and toxicology. J. Cancer Res. Clin. Oncol. 116(6):557-562.
  • Emura, M., Jacob, J., Knebel, J. W., Aufderheide, M., Grimmer, G., and Mohr, U. 1995. Establishment of in vitro systems from the respiratory tract of different species for the investigation of PAH metabolism. Exp. Toxicol. Pathol. 47(6):434-439.
  • Gupta, M. P., Khanduja, K. L., and Sharma, R. R. 1988. Effect of cigarette smoke inhalation on antioxidant enzymes and lipid peroxidation in the rat. Toxicol. Lett. 4(2):107-114.
  • Helen, A., and Vijayammal, P. L. 1997. Effect of vitamin A supplementation on cigarette smoke-induced lipid peroxidation. Vet. Hum. Toxicol. 39(1):18-21.
  • Heyder, J., Gebhart, J., Rudolf, G., Schiller, C. F., and Stanhlhofen, W. 1986. Deposition of particles in the human respiratory tract in the size range 0.005-15 µm. J. Aerosol Sci. 17(5):811-825.
  • Higenbottam, T., Feyeraband, C., and Clark, T. J. 1980. Cigarette smoke inhalation and the acute airway response. Thorax 35(4):246-254.
  • Hirafuji, M., Sakakibara, M., Endo, T., Murakami, S., Mori, Y., Sagai, M., and Minami, M. 1995. Biological effects of diesel exhaust particles (DEP) on tissues and cells isolated from respiratory tracts of guinea pigs. Res. Commun. Mol. Pathol. Pharmacol. 90(2):221-233.
  • Hockele, V., Kruger, E., Krug, H. F., and Seidel, A. 1998. In vitro effects of nitrogen dioxide on the release of nitric oxide by bovine alveolar macrophages. Toxicol. Lett. 96-97:53-57.
  • Hoffmann, D., and Wynder, E. L. 1986. Chemical constituents and bioactivity of tobacco smoke. IARC Sci. Publ. (74):145-165.
  • Jabbour, A. J., Altman, L. C., Wight, T. N., and Luchtel, D. L. 1998. Ozone alters the distribution of beta 1 integrins in cultured primate bronchial epithelial cells. Am. J. Respir. Cell Mol. Biol. 19(3):357-365.
  • Knebel, J. W., Ritter, D., and Aufderheide, M. 2002. Exposure of human lung cells to native diesel motor exhaust--Development of an optimized in vitro test strategy. Toxicol. In Vitro 16(2):185-192.
  • Lang, D. S., Jorres, R. A., Mucke, M., Siegfried, W., and Magnussen, H. 1998. Interactions between human bronchoepithelial cells and lung fibroblasts after ozone exposure in vitro. Toxicol. Lett. 96-97:13-24.
  • Li, X. Y., Donaldson, K., Rahman, L., and MacNee, W. 1994. An investigation of the role of glutathione in increased epithelial permeability induced by cigarette smoke in vivo and in vitro. Am. J. Respir. Crit. Care Med. 149(6):1518-1525.
  • Li, X. Y., Rahman, I., Donaldson, K., and MacNee, W. 1996. Mechanisms of cigarette smoke induced increased airspace permeability. Thorax 51(5):465-471.
  • Lombardo, T., and Carreno, L. 1987. Relationship of type A behavior pattern in smokers to carbon monoxide exposure and smoking topography. Health Psychol. 6(5):445-452.
  • Maranzana, A., and Mehlhorn, R. J. 1998. Loss of glutathione, ascorbate recycling, and free radical scavenging in human erythrocytes exposed to filtered cigarette smoke. Arch. Biochem. Biophys. 350(2):169-182.
  • Mogel, M., Kruger, E., Krug, H. F., and Seidel, A. 1998. A new coculture-system of bronchial epithelial and endothelial cells as a model for studying ozone effects on airway tissue. Toxicol. Lett. 96-97:25-32.
  • Morrison, D., Rahman, I., Lannan, S., and MacNee, W. 1999. Epithelial permeability, inflammation, and oxidant stress in the air spaces of smokers. Am. J. Respir. Crit. Care Med. 159(2):473-479.
  • Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65(1-2):55-63.
  • Muckter, H., Zwing, M., Bader, S., Marx, T., Doklea, E., Leibl, B., Fichtl, B., and Georgieff, M. 1998. A novel apparatus for the exposure of cultured cells to volatile agents. J. Pharmacol. Toxicol. Methods 40(2):63-69.
  • Muller, T., and Gebel, S. 1998. The cellular stress response induced by aqueous extracts of cigarette smoke is critically dependent on the intracellular glutathione concentration. Carcinogenesis 19(5):797-801.
  • Ochiai, A., Emura, M., Riebe-Imre, M., Mohr, U., Hilfrich, J., Tahara, E., and Dungworth, D. L. 1991. Secretory differentiation and cell type identification of a human fetal bronchial epithelial cell line (HFBE). Virchows Arch. B Cell Pathol. Incl. Mol. Pathol. 61(3):217-226.
  • Pace, D. M., Landolt, P. A., and Altonomos, B. T. 1969. Effects of ozone on cells in vitro. Arch. Environ. Health 18(2):165-170.
  • Park, E. M., Park, Y. M., and Gwak, Y. S. 1998. Oxidative damage in tissues of rats exposed to cigarette smoke. Free Radical Biol. Med. 25(1):79-86.
  • Pryor, W. A. 1992. Biological effects of cigarette smoke, wood smoke, and the smoke from plastics: The use of electron spin resonance. Free Radical Biol. Med. 13(6):659-676.
  • Rahman, I., Li, X. Y., Donaldson, K., Harrison, D. J., and MacNee, W. 1995. Glutathione homeostasis in alveolar epithelial cells in vitro and lung in vivo under oxidative stress. Am. J. Physiol. 269(3 Pt. 1): L285-L292.
  • Rahman, I., Bel, A., Mulier, B., Donaldson, K., and MacNee, W. 1998. Differential regulation of glutathione by oxidants and dexamethasone in alveolar epithelial cells. Am. J. Physiol. 275(1 Pt.1):L80-L86.
  • Rasmussen, R. E. 1984. In vitro systems for exposure of lung cells to NO2 and O3. J. Toxicol. Environ. Health 13(2-3):397-411.
  • Ritter, D., Knebel, J. W., Aufderheide, M., and Mohr, U. 1999, Development of a cell culture model system for routine testing of substances inducing oxidative stress. In Vitro Toxicol. 13(4/5):745-751.
  • Ritter, D., Knebel, J. W., and Aufderheide, M. 2001. In vitro exposure of isolated cells to native gaseous compounds--Development and validation of an optimized system for human lung cells. Exp. Toxicol. Pathol. 53:373-386.
  • Rodenstein, D. O., and Stanescu, D. C. 1985. Pattern of inhalation of tobacco smoke in pipe, cigarette, and never smokers. Am. Rev. Respir. Dis. 132(3):628-632.
  • Rusznak, C., Devalia, J. L., Sapsford, R. J., and Davies, R. J. 1996. Ozone-induced mediator release from human bronchial epithelial cells in vitro and the influence of nedocromil sodium. Eur. Respir. J. 9(11):2298-2305.
  • Schlesinger, R. B. 1985. Comparative deposition of inhaled aerosols in experimental animals and humans: A review. J. Toxicol. Environ. Health 15(2):197-214.
  • Slater, T. F., Sawyer, B., and Sträuli, U. 1963. Studies on succinate-tetrazolium reductase systems: III. Points of coupling four different tetrazolium salts. Biochim. Biophys. Acta 77:383-393.
  • Tietze, F. 1969. Enzymic method for quantitative determination of nanogram amounts of total and oxidized-glutathione: Applications to mammalian blood and other tissues. Anal. Biochem. 27(3):502-522.
  • Tu, B., Wallin, A., Moldeus, P., and Cotgreave, I. A. 1995. Cytotoxicity of NO, gas to cultured human and murine cells in an inverted monolayer exposure system. Toxicology 96(1):7-18.
  • Uejima, Y., Fukuchi, Y., Nagase, T., Matsuse, T., Yamaoka, M., Tabata, R., and Orimo, H. 1990. Influences of inhaled tobacco smoke on the senescence accelerated mouse (SAM). Eur. Respir. J. 3(9):1029-1036.
  • Valberg, P. A. 1985. Determination of retained lung dose. In Toxicology of inhaled materials, eds. H. P. Witschi and J. D. Brain, vol. 75, pp. 57-94. Berlin, Springer Verlag.
  • Van der Zee, J., Tijssen Christianse, K., Dubbelman, T. M., and Van Steveninck, J. 1987. The influence of ozone on human red blood cells. Comparison with other mechanisms of oxidative stress. Biochim. Biophys. Acta 924(1):111-118.
  • Vandeputte, C., Guizon, I., Genestie-Denis, I., Vannier, B., and Lorenzon, G. 1994. A microtiter plate assay for total glutathione and glutathione disulfide contents in cultured/isolated cells: Performance study of a new miniaturized protocol. Cell Biol. Toxicol. 10(5-6):415-421.
  • Voisin, C., Aerts, C., Houdret, J. L., and Tonnel, A. B. 1974. Effect of NO2 on alveolar macrophages. In Reactions bronchopulmonaires aux pollutants atmospheriques, ed. C. Voisin, pp. 273-288, Paris: INSERM.
  • Voisin, C., Aerts, C., Jakubczak, E., Houdret, J. L., and Tonnel, T. B. 1977. Effects of nitrogen dioxide on alveolar macrophages surviving in the gas phase. A new experimental mode for the study of in vitro cytotoxicity of toxic gases. Bull. Eur. Physiopathol. Respir. 13(1):137-144.
  • Wallaert, B., Gosset, P., Boitelle, A., and Tonnel, A. B. 1996. In vitro assessment of environmental toxicology using alveolar cells as target. Cell Biol. Toxicol. 12(4-6):251-256.
  • Winkelmeier, P., Glauner, B., and Lindl, T. 1993. Quantification of cytotoxicity by cell volume and cell proliferation. ATLA 21:269-280.
  • Zamora, P. O., Gregory, R. E., Li, A. P., and Brooks, A. L. 1986. An in vitro model for the exposure of lung alveolar epithelial cells to toxic gases. J. Environ. Pathol. Toxicol. Oncol. 7(1-2):159-168.

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