Increased trypsin resilience in aqueous-acetonitrile environment when immobilized on glyoxyl-agarose may improve its applicability

References

• Amalia S, Angga SC, Iftitah ED, Septiana D, Anggraeny BOD, Hasanah AN, Sabarudin, A, Warsito. 2021. Immobilization of trypsin onto porous methacrylate-based monolith for flow-through protein digestion and its potential application to chiral separation using liquid chromatography. Heliyon. 7(8):e07707.
   PubMed Web of Science ®Google Scholar
• Batra R, Gupta MN. 1994. Enhancement of enzyme activity in aqueous-organic solvent mixtures. Biotechnol Lett. 16(10):1059–1064.
   Web of Science ®Google Scholar
• Bolivar JM, Woodley JM, Fernandez-Lafuente R. 2022. Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization. Chem Soc Rev. 51(15):6251–6290.
   PubMed Web of Science ®Google Scholar
• Bradford MM. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72(1-2):248–254.
   PubMed Web of Science ®Google Scholar
• Braham SA, Morellon-Sterling R, de Andrades D, Rodrigues RC, Siar E-H, Aksas A, Pedroche J, Millán MC, Fernandez-Lafuente R. 2021. Effect of tris buffer in the intensity of the multipoint covalent immobilization of enzymes in glyoxyl-agarose beads. Appl Biochem Biotechnol. 193(9):2843–2857.
   PubMed Web of Science ®Google Scholar
• Crowell AMJ, Stewart EJ, Take ZS, Doucette AA. 2013. Critical assessment of the spectroscopic activity assay for monitoring trypsin activity in organic-aqueous solvent. Anal Biochem. 435(2):131–136.
   PubMed Web of Science ®Google Scholar
• Fernández-Lorente G, Lopez-Gallego F, Bolivar JM, Rocha-Martin J, Moreno-Perez S, Guisan JM. 2015. Immobilization of proteins on glyoxyl activated supports: dramatic stabilization of enzymes by multipoint covalent attachment on pre-existing supports. COC. 19(999):1–1.
   Google Scholar
• Garcia-Galan C, Berenguer-Murcia A, Fernandez-Lafuente R, Rodrigues RC. 2011. Potential of different enzyme immobilization strategies to improve enzyme performance. Adv Synth Catal. 353(16):2885–2904.
   Web of Science ®Google Scholar
• Gobom J, Nordhoff E, Ekman R, Roepstorff P. 1997. Rapid micro-scale proteolysis of proteins for MALDI-MS peptide mapping using immobilized trypsin. Int J Mass Spectrom Ion Process. 169-170:153–163.
   Web of Science ®Google Scholar
• Guisán JM. 1988. Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes. Enzyme Microb Technol. 10(6):375–382.
   Web of Science ®Google Scholar
• Liang L-H, Yang Y, Geng S, Cheng X, Yu H-L, Liu C-C, Liu S-L. 2021. Rapid differential detection of abrin isoforms by an acetonitrile- and ultrasound-assisted on-bead trypsin digestion coupled with LC-MS/MS analysis. Toxins. 13(5):358.
   PubMed Web of Science ®Google Scholar
• Liu C, Saeki D, Matsuyama H. 2017. A novel strategy to immobilize enzymes on microporous membranes via dicarboxylic acid halides. RSC Adv. 7(76):48199–48207.
   Web of Science ®Google Scholar
• Liu C-C, Liang L-H, Yang Y, Yu H-L, Yan L, Li X-S, Chen B, Liu S-L, Xi H-L. 2021. Direct acetonitrile-assisted trypsin digestion method combined with LC-MS/MS-targeted peptide analysis for unambiguous identification of intact ricin. J Proteome Res. 20(1):369–380.
   PubMed Web of Science ®Google Scholar
• Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R. 2007. Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb Technol. 40(6):1451–1463.
   Web of Science ®Google Scholar
• Mateo C, Palomo JM, Fuentes M, Betancor L, Grazu V, López-Gallego F, Pessela BC, Hidalgo A, Fernández-Lorente G, Fernández-Lafuente R, et al. 2006. Glyoxyl agarose: a fully inert and hydrophilic support for immobilization and high stabilization of proteins. Enzyme Microb Technol. 39(2):274–280.
   Web of Science ®Google Scholar
• Meng Y, Yuan Y, Zhu Y, Guo Y, Li M, Wang Z, Pu X, Jiang L. 2013. Effects of organic solvents and substrate binding on trypsin in acetonitrile and hexane media. J Mol Model. 19(9):3749–3766.
   PubMed Web of Science ®Google Scholar
• Morellon-Sterling R, Siar E-H, Braham SA, de Andrades D, Pedroche J, Millán MDC, Fernandez-Lafuente R. 2021. Effect of amine length in the interference of the multipoint covalent immobilization of enzymes on glyoxyl agarose beads. J Biotechnol. 329:128–142.
   PubMed Web of Science ®Google Scholar
• Naveed M, Nadeem F, Mehmood T, Bilal M, Anwar Z, Amjad F. 2021. Protease—a versatile and ecofriendly biocatalyst with multi-industrial applications: an updated review. Catal Lett. 151(2):307–323.
   Web of Science ®Google Scholar
• Oyler JM, Tran BQ, David PA. 2021. Rapid denaturing organic digestion method for targeted protein identification and characterization. Anal Chem. 93(12):5046–5053.
   PubMed Web of Science ®Google Scholar
• Pecquet S, Bovetto L, Maynard F, Fritsché R. 2000. Peptides obtained by tryptic hydrolysis of bovine β-lactoglobulin induce specific oral tolerance in mice. J Allergy Clin Immunol. 105(3):514–521.
   PubMed Web of Science ®Google Scholar
• Pessato TB, Tavano OL. 2015. Hydrolysis of casein and β -lactoglobulin by immobilized papain after pre-treatment with immoblized trypsin. Acta Alimentaria. 44, 4(4):570–577.
   Web of Science ®Google Scholar
• Qiao J, Kim JY, Wang YY, Qi L, Wang FY, Moon WH. 2016. Trypsin immobilization in ordered porous polymer membranes for effective protein digestion. Anal Chim Acta. 906:156–164.
   PubMed Web of Science ®Google Scholar
• Rodrigues RC, Berenguer-Murcia Á, Carballares D, Morellon-Sterling R, Fernandez-Lafuente R. 2021. Stabilization of enzymes via immobilization: multipoint covalent attachment and other stabilization strategies. Biotechnol Adv. 52:107821.
   PubMed Web of Science ®Google Scholar
• Royer GP, Uy R. 1973. Evidence for the induction of a conformational change of bovine trypsin by a specific substrate at pH 8.0. J Biol Chem. 248(7):2627–2629.
   PubMed Web of Science ®Google Scholar
• Russell WK, Park ZY, Russell DH. 2001. Proteolysis in mixed organic-aqueous solvent systems: applications for peptide mass mapping using mass spectrometry. Anal Chem. 73(11):2682–2685.
   PubMed Web of Science ®Google Scholar
• Santos JCS, Rueda N, Barbosa O, Millán-Linares MC, Pedroche J, del Mar Yuste M, Goncalves LRB, Fernandez-Lafuente R. 2015. Bovine trypsin immobilization on agarose activated with divinylsulfone: improved activity and stability via multipoint covalent attachment. J Mol Catal B: enzym. 117:38–44.
   Google Scholar
• Sheldon RA, Van Pelt S. 2013. Enzyme immobilisation in biocatalysis: why, what and how. Chem Soc Rev. 42(15):6223–6235.
   PubMed Web of Science ®Google Scholar
• Simon LM, Kotormán M, Garab G, Laczkó I. 2001. Structure and activity of α-chymotrypsin and trypsin in aqueous organic media. Biochem Biophys Res Commun. 280(5):1367–1371.
   PubMed Web of Science ®Google Scholar
• Sneha JP, Pandey JP, Pandey DM. 2022. Evaluating the role of trypsin in silk degumming: an in silico approach. J Biotechnol. 359:35–47.
   PubMed Web of Science ®Google Scholar
• Statsoft 2007. Statistics computer program for windows. Vol. 1. Software. Tulsa: StatSoft Inc.
   Google Scholar
• Sun X, Cai X, Wang RQ, Xiao J. 2015. Immobilized trypsin on hydrophobic cellulose decorated nanoparticles shows good stability and reusability for protein digestion. Anal Biochem. 477:1, 21–27.
   Web of Science ®Google Scholar
• Tavano OL, Berenguer-Murcia A, Secundo F, Fernandez-Lafuente R. 2018. Biotechnological applications of proteases in food technology. Compr Rev Food Sci Food Saf. 17(2):412–436.
   PubMed Web of Science ®Google Scholar
• Tavano OL. 2013. Protein hydrolysis using proteases: an important tool for food biotechnology. J. Mol. Catal.B-Enzym. 90:1–11.
   Web of Science ®Google Scholar
• Ulug SK, Jahandideh F, Wu J. 2021. Novel technologies for the production of bioactive peptides. Trends Food Sci Technol. 108:27–39.
   Web of Science ®Google Scholar
• Wojtkiewicz M, Berg Luecke L, Kelly MI, Gundry RL. 2021. Facile preparation of peptides for mass spectrometry analysis in bottom-up proteomics workflows. Curr Protoc. 1(3):e85.
   PubMedGoogle Scholar
• Yeung AWK, Tzvetkov NT, Gupta VK, Gupta SC, Orive G, Bonn GK, Fiebich B, Bishayee A, Efferth T, Xiao J, et al. 2019. Current research in biotechnology: exploring the biotech forefront. Current Research in Biotechnology. 1:34–40.
   Google Scholar