Ophrys fusca and Ophrys dyris (Orchidaceae) – constancy of tetraploidy amongst populations in Central Portugal

References

• Aedo, C. & Herreo, A., eds. 2005. Orchidaceae, in Castroviejo, S., ed. Flora Iberica. Plantas vasculares de la Peninsula Ibérica e Islas Baleares (Vol. XXI. Smilacaceae-Orchidaceae). Pp 165–195. Madrid: Real Jardín Botánico.
   Google Scholar
• Amich, F., García-Barriuso, M. & Bernardos, S. 2007. Polyploidy and speciation in the orchid flora of the Iberian Peninsula. Botanica Helvetica, 117: 143–157. doi: 10.1007/s00035-007-0804-0
   Google Scholar
• Anderson, E. 1949. Introgressive hybridisation. New York: John Wiley & Sons, Inc.
   Google Scholar
• Bernardos, S., Amich, F. & Gallego, F. 2003. Karyological and taxonomic notes on Ophrys L. (Orchidoideae, Orchidaceae) from the Iberian Peninsula. Botanical Journal of the Linnean Society, 142: 395–406. doi: 10.1046/j.1095-8339.2003.00198.x
   Web of Science ®Google Scholar
• Bernardos, S., Crespi, A., Del Rey, F. & Amich, F. 2005. The section Pseudophrys (Ophrys, Orchidaceae) in the Iberian Peninsula: a morphometric and molecular analysis. Botanical Journal of the Linnean Society, 148: 359–375. doi: 10.1111/j.1095-8339.2005.00403.x
   Web of Science ®Google Scholar
• Burton, T. L. & Husband, B. C. 2000. Fitness differences among diploids, tetraploids, and their triploid progeny in Chamerion angustifolium: mechanisms of inviability and implications for polyploid evolution. Evolution, 54: 1182–1191. doi: 10.1111/j.0014-3820.2000.tb00553.x
   PubMed Web of Science ®Google Scholar
• Carroll, C. P. & Borrill, M. 1965. Tetraploid hybrids from crosses between diploid and tetraploid Dactylis and their significance. Genetica, 36: 65–82. doi: 10.1007/BF01557144
   PubMed Web of Science ®Google Scholar
• Cotrim, H., Monteiro, F., Sousa, E., Pinto, M. J. & Fay, M. F. 2016. Marked hybridization and introgression in Ophrys sect. Pseudophrys in the western Iberian Peninsula. American Journal of Botany, 103: 677–691. doi: 10.3732/ajb.1500252
   PubMed Web of Science ®Google Scholar
• Cotrim, H., Monteiro, F., Sousa, E. S., Fay, M. F., Chase, M. W. & Pais, M. S. 2009. Isolation and characterization of novel polymorphic nuclear microsatellite markers from Ophrys fusca (Orchidaceae) and cross-species amplification. [Technical note]. Conservation Genetics, 10: 739–742. doi: 10.1007/s10592-008-9634-x
   Google Scholar
• D'Emerico, S., Pignone, D., Bartolo, G., Pulvirenti, S., Terrasi, C., Stuto, S. & Scrugli, A. 2005. Karyomorphology, heterochromatin patterns and evolution in the genus Ophrys (Orchids). Botanical Journal of the Linnean Society, 148: 87–99. doi: 10.1111/j.1095-8339.2005.00393.x
   Web of Science ®Google Scholar
• Delforge, P. ed. 2002. Guía de las Orquideas de España y Europa, Norte de Africa y Próximo Oriente. Barcelona: Lynx Editions.
   Google Scholar
• Devey, D. S., Bateman, R. M., Fay, M. F. & Hawkins, J. A. 2008. Friends or relatives? Phylogenetics and species delimitation in the controversial European orchid genus Ophrys. Annals of Botany, 101: 1–18.
   PubMed Web of Science ®Google Scholar
• Doležel, J., Greilhuber, J. & Suda, J. 2007. Estimation of nuclear DNA content in plants using flow cytometry. [10.1038/nprot.2007.310]. Nature Protocols, 2: 2233–2244. doi: 10.1038/nprot.2007.310
   PubMed Web of Science ®Google Scholar
• Ebihara, A., Ishikawa, H., Matsumoto, S., Lin, S.-J., Iwatsuki, K., Takamiya, M., Watano, Y. & Ito, M. 2005. Nuclear DNA, chloroplast DNA, and ploidy analysis clarified biological complexity of the Vandenboschia radicans complex (Hymenophyllaceae) in Japan and adjacent areas. American Journal of Botany, 92: 1535–1547. doi: 10.3732/ajb.92.9.1535
   PubMed Web of Science ®Google Scholar
• García-Barriuso, M., Bernardos, S. & Amich, F. 2010. Chromosomal evolution in Mediterranean species of Ophrys sect. Pseudophrys (Orchidaceae): an analysis of karyotypes and polyploidy. Taxon, 59: 525–537.
   Web of Science ®Google Scholar
• Greilhuber, J. & Ehrendorfer, F. 1975. Chromosome numbers and evolution in Ophrys (Orchidaceae). Plant Systematics and Evolution, 124: 125–138. doi: 10.1007/BF00985498
   Web of Science ®Google Scholar
• Kullenberg, B. 1961. Studies in Ophrys pollination. Zoologiska Bidrag från Uppsala, 34: 1–340.
   Google Scholar
• Lowe, M. R. & Tyteca, D. 2012. Two new Ophrys species from Portugal. Journal Europaischer Orchideen: Mitteilungsblatt des AHO Baden-Württemberg, 44: 207–229.
   Google Scholar
• Paulus, H. F. & Gack, C. 1990. Pollinators as prepollinating isolation factors: evolution and speciation in Ophrys pollination (Orchidaceae). Israel Journal of Botany, 39: 43–79.
   Web of Science ®Google Scholar
• Pederson, H. A. & Faurholdt, N. ed. 2007. Ophrys. The Bee Orchids of Europe. Kew: Kew Publishing.
   Google Scholar
• Petit, C., Bretagnolle, F. & Felber, F. 1999. Evolutionary consequences of diploid-polyploid hybrid zones in wild species. Trends in Ecology and Evolution, 14: 306–311. doi: 10.1016/S0169-5347(99)01608-0
   PubMed Web of Science ®Google Scholar
• Pellicer, J., Clermont, S., Houston, L., Rich, T.C.G. & Fay, M.F. 2012. Cytotype diversity in the Sorbus complex (Rosaceae) in Britain: sorting out the puzzle. Annals of Botany, 110: 1185–1193. doi: 10.1093/aob/mcs185
   PubMed Web of Science ®Google Scholar
• Pellicer, J. & Leitch, I. J. 2014. The application of flow cytometry for estimating genome size and ploidy level in plants. In Besse, P., ed. Methods in Molecular Biology. New York: Springer, pp 279–307.
   Google Scholar
• Rodriguez, D. J. 1996. A model for the establishment of polyploidy in plants. The American Naturalist, 137: 43–46.
   Google Scholar
• Sabara, H. A., Kron, P. & Husband, B. C. 2013. Cytotype coexistence leads to triploid hybrid production in a diploid–tetraploid contact zone of Chamerion angustifolium (Onagraceae). American Journal of Botany, 100: 962–970. doi: 10.3732/ajb.1200583
   PubMed Web of Science ®Google Scholar
• Scopece, G., Musacchio, A., Widmer, A. & Cozzolino, S. 2007. Patterns of reproductive isolation in Mediterranean deceptive orchids. Evolution, 61: 2623–2642. doi: 10.1111/j.1558-5646.2007.00231.x
   PubMed Web of Science ®Google Scholar
• Soliva, M., Kocyan, A. & Widmer, A. 2001. Molecular phylogenetics of the sexually deceptive orchid genus Ophrys (Orchidaceae) based on nuclear and chloroplast DNA sequences. Molecular Phylogenetics and Evolution, 20: 78–88. doi: 10.1006/mpev.2001.0953
   PubMed Web of Science ®Google Scholar
• Stebbins, G. L. 1971. Chromosomal evolution in higher plants. London: Edward Arnold Ltd.
   Google Scholar
• Stebbins, G. L. & Ferlan, L. 1956. Population variability, hybridization and introgression in some species of Ophrys. Evolution, 10: 32–46.
   Web of Science ®Google Scholar
• Trávníček, P., Ponert, J., Urfus, T., Jersáková, J., Vrána, J., Hřibová, E., Doležel, J. & Suda, J. 2015. Challenges of flow-cytometric estimation of nuclear genome size in orchids, a plant group with both whole-genome and progressively partial endoreplication. Cytometry Part A, 87: 958–966. doi: 10.1002/cyto.a.22681
   PubMed Web of Science ®Google Scholar
• Van Santen, E., Hugessen, P. M. & Casler, M. D. 1991. Identification and frequency of tetraploid progeny from 2x-4x and 4x-2x crosses in Dactylis. Genome, 34: 273–278. doi: 10.1139/g91-043
   Web of Science ®Google Scholar
• Yakimowski, S. B. & Rieseberg, L. H. 2014. The role of homoploid hybridization in evolution: a century of studies synthesizing genetics and ecology. American Journal of Botany, 101: 1247–1258. doi: 10.3732/ajb.1400201
   PubMed Web of Science ®Google Scholar