Advanced search
Publication Cover
Small GTPases Volume 14, 2023 - Issue 1
Submit an article Journal homepage
1,024
Views
0
CrossRef citations to date
0
Altmetric
Brief Report

Serine phosphorylation of the RhoGEF Trio stabilizes endothelial cell-cell junctions

Anna E. Daniela Department of Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The NetherlandsView further author information
,
Werner J. van der Meerb Department of Medical Biochemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, The NetherlandsView further author information
,
Lynn Westera Department of Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The NetherlandsView further author information
,
Vivian de Waardb Department of Medical Biochemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, The NetherlandsView further author information
,
Maartje van den Biggelaara Department of Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The NetherlandsView further author information
&
Jaap D. van Buula Department of Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands;b Department of Medical Biochemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands;c Section Molecular Cytology at Swammerdam Institute for Life Sciences, Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam, The NetherlandsCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0003-0054-7949View further author information
ORCID Icon
Pages 45-54 | Received 01 Jun 2023, Accepted 25 Jul 2023, Published online: 01 Aug 2023

References

  • Burridge K, Wennerberg K. Rho and rac take center stage. Cell. 2004;116(2):167–179. doi: 10.1016/S0092-8674(04)00003-0
  • Bos JL, Rehmann H, Wittinghofer A. Gefs and GAPs: critical elements in the control of small G proteins. Cell. 2007;129(5):865–877. doi: 10.1016/j.cell.2007.05.018
  • Rossman KL, Der CJ, Sondek J. GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors. Nat Rev Mol Cell Biol. 2005;6(2):167–180. doi: 10.1038/nrm1587
  • Wennerberg K, Der CJ. Rho-family GTPases: it’s not only Rac and Rho (and I like it). J Cell Sci. 2004;117(8):1301–1312. doi: 10.1242/jcs.01118
  • Bar-Sagi D, Rotin D, Batzer A, et al. SH3 domains direct cellular localization of signaling molecules. Cell. 1993;74(1):83–91. doi: 10.1016/0092-8674(93)90296-3
  • van Rijssel J, Kroon J, Hoogenboezem M, et al. The Rho-guanine nucleotide exchange factor Trio controls leukocyte transendothelial migration by promoting docking structure formation. Mol Biol Cell. 2012;23(15):2831–2844.
  • van Rijssel J, Hoogenboezem M, Wester L, et al. The N-terminal DH-PH domain of Trio induces cell spreading and migration by regulating lamellipodia dynamics in a Rac1-dependent fashion. PLoS One. 2012;7:e29912.
  • van Rijssel J, van Buul JD. Cell Adh Migr. 2012Nov–Dec;6(6):482–487.
  • Debant A, Serra-Pages C, Seipel K, et al. The multidomain protein Trio binds the LAR transmembrane tyrosine phosphatase, contains a protein kinase domain, and has separate rac-specific and rho-specific guanine nucleotide exchange factor domains. Proc Natl Acad Sci USA. 1996;93(11):5466–5471. doi: 10.1073/pnas.93.11.5466
  • DeGeer J, Boudeau J, Schmidt S, et al. Tyrosine phosphorylation of the rho guanine nucleotide exchange factor trio regulates netrin-1/DCC-Mediated cortical axon outgrowth. Mol Cell Biol. 2013;33(4):739–751. doi: 10.1128/MCB.01264-12
  • Timmerman I, Heemskerk N, Kroon J, et al. A local VE-cadherin and Trio-based signaling complex stabilizes endothelial junctions through Rac1. J Cell Sci. 2015;128(16):3041–3054.
  • van den Biggelaar M, Hernandez-Fernaud JR, van den Eshof BL, et al. Quantitative phosphoproteomics unveils temporal dynamics of thrombin signaling in human endothelial cells. Blood. 2014;123(12):e22–e36. doi: 10.1182/blood-2013-12-546036
  • Feig LA. Tools of the trade: use of dominant-inhibitory mutants of Ras-family GTPases. Nat Cell Biol. 1999;1(2):E25–E27. doi: 10.1038/10018
  • Garcia-Mata R, Wennerberg K, Arthur WT, et al. Analysis of activated GAPs and GEFs in cell lysates. Methods Enzymol. 2006;406:425–437.
  • Bustelo XR. Regulation of Vav proteins by intramolecular events. Front Biosci. 2002;7(4):d24–d30. doi: 10.2741/A766
  • Aghazadeh B, Lowry WE, Huang XY, et al. Structural basis for relief of autoinhibition of the dbl homology domain of proto-oncogene vav by tyrosine phosphorylation. Cell. 2000;102(5):625–633. doi: 10.1016/S0092-8674(00)00085-4
  • Barreira M, Fabbiano S, Couceiro JR, et al. The C-Terminal SH3 domain contributes to the intramolecular inhibition of Vav family proteins. Sci. 2014;7(321):ra35. doi: 10.1126/scisignal.2004993
  • Lopez-Lago M, Lee H, Cruz C, et al. Tyrosine Phosphorylation Mediates Both Activation and Downmodulation of the Biological Activity of Vav. Mol Cell Biol. 2000;20(5):1678–1691. doi: 10.1128/MCB.20.5.1678-1691.2000
  • Yu B, Martins IR, Li P, et al. Structural and energetic mechanisms of cooperative autoinhibition and activation of Vav1. Cell. 2010;140(2):246–256. doi: 10.1016/j.cell.2009.12.033
  • Chhatriwala MK, Betts L, Worthylake DK, et al. The DH and PH domains of trio coordinately engage Rho GTPases for their efficient activation. J Mol Biol. 2007;368(5):1307–1320. doi: 10.1016/j.jmb.2007.02.060
  • Skowronek KR, Guo F, Zheng Y, et al. The C-terminal basic tail of RhoG assists the guanine nucleotide exchange factor trio in binding to phospholipids. J Biol Chem. 2004;279(36):37895–37907. doi: 10.1074/jbc.M312677200
  • Xin X, Ferraro F, Back N, et al. Cdk5 and Trio modulate endocrine cell exocytosis. J Cell Sci. 2004;117(20):4739–4748. doi: 10.1242/jcs.01333
  • Ando K, Fukuhara S, Moriya T, et al. Rap1 potentiates endothelial cell junctions by spatially controlling myosin II activity and actin organization. J Cell Bio. 2013;202(6):901–916. doi: 10.1083/jcb.201301115

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.