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Brief Report

Housekeeping gene expression variability in differentiating and non-differentiating 3T3-L1 cells

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Article: 2235081 | Received 04 Jun 2023, Accepted 06 Jul 2023, Published online: 20 Jul 2023

References

  • Blüher M. Obesity: global epidemiology and pathogenesis. Nat Rev Endocrinol. 2019;15(5):288–13. doi: 10.1038/s41574-019-0176-8
  • Anandacoomarasamy A, Caterson I, Sambrook P, et al. The impact of obesity on the musculoskeletal system. Int J Obes. 2008;32(2):211–222. doi: 10.1038/sj.ijo.0803715
  • Czernichow S, Kengne AP, Stamatakis E, et al. Body mass index, waist circumference and waist-hip ratio: which is the better discriminator of cardiovascular disease mortality risk? evidence from an individual-participant meta-analysis of 82 864 participants from nine cohort studies. Obes Rev. 2011;12(9):680–687. doi: 10.1111/j.1467-789X.2011.00879.x
  • Sarma S, Sockalingam S, Dash S. Obesity as a multisystem disease: trends in obesity rates and obesity-related complications. Diab Obes Metab. 2021;23(S1):3–16. doi: 10.1111/dom.14290
  • Coronado-Ferrer S, Ferrer-Sapena A, Aleixandre-Benavent R, et al. Global trends in scientific research on pediatric obesity. Int J Environ Res Public Health. 2022;19(3):1251. doi: 10.3390/ijerph19031251
  • Zhao N, Tao K, Wang G, et al. Global obesity research trends during 1999 to 2017: a bibliometric analysis. Medicine (Baltimore). 2019;98(4):e14132. doi: 10.1097/MD.0000000000014132
  • Green H, Kehinde O. Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells. Cell. 1976;7(1):105–113. doi: 10.1016/0092-8674(76)90260-9
  • Green H, Meuth M. An established pre-adipose cell line and its differentiation in culture. Cell. 1974;3(2):127–133. doi: 10.1016/0092-8674(74)90116-0
  • Negrel R, Grimaldi P, Ailhaud G. Establishment of preadipocyte clonal line from epididymal fat pad of ob/ob mouse that responds to insulin and to lipolytic hormones. Proc Natl Acad Sci, USA. 1978;75(12):6054–6058. doi: 10.1073/pnas.75.12.6054
  • Taylor SM, Jones PA. Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine. Cell. 1979;17(4):771–779. doi: 10.1016/0092-8674(79)90317-9
  • Doi H, Masaki N, Takahashi H, et al. A new preadipocyte cell line, AP-18, established from adult mouse adipose tissue. Tohoku J Exp Med. 2005;207(3):209–216. doi: 10.1620/tjem.207.209
  • Wolins NE, Quaynor BK, Skinner JR, et al. OP9 mouse stromal cells rapidly differentiate into adipocytes: characterization of a useful new model of adipogenesis. J Lipid Res. 2006;47(2):450–460. doi: 10.1194/jlr.D500037-JLR200
  • Huang E, Bi Y, Jiang W, et al. Conditionally immortalized mouse embryonic fibroblasts retain proliferative activity without compromising multipotent differentiation potential. PLoS One. 2012;7(2):e32428. doi: 10.1371/journal.pone.0032428
  • Dufau J, Shen JX, Couchet M, et al. In vitro and ex vivo models of adipocytes. Am J Physiol Cell Physiol. 2021;320(5):C822–C841. doi: 10.1152/ajpcell.00519.2020
  • Rosen ED, MacDougald OA. Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol. 2006;7(12):885–896. doi: 10.1038/nrm2066
  • Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol. 2000;25(2):169–193. doi: 10.1677/jme.0.0250169
  • Smith CJ, Osborn AM. Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. FEMS Microbiol Ecol. 2009;67(1):6–20. doi: 10.1111/j.1574-6941.2008.00629.x
  • Huggett J, Dheda K, Bustin S, et al. Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 2005;6(4):279–284. doi: 10.1038/sj.gene.6364190
  • Bustin SA, Benes V, Garson JA, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009;55(4):611–622. doi: 10.1373/clinchem.2008.112797
  • Chapman JR, Waldenström J. With reference to reference genes: a systematic review of endogenous controls in gene expression studies. PLoS One. 2015;10(11):e0141853. doi: 10.1371/journal.pone.0141853
  • Derks NM, Müller M, Gaszner B, et al. Housekeeping genes revisited: different expressions depending on gender, brain area and stressor. Neuroscience. 2008;156(2):305–309. doi: 10.1016/j.neuroscience.2008.07.047
  • Anbazhagan A, Sridharan P, Pratheesh P. Validation of suitable housekeeping genes for 3T3-L1 derived adipocytes cultured in obesity mimicking conditions and RAW 264.7 macrophage cells lines in hypoxic and normoxic conditions. Biocatal Agric Biotechnol. 2019;17:620–627. doi: 10.1016/j.bcab.2019.01.006
  • Arsenijevic T, Grégoire F, Delforge V, et al. Murine 3T3-L1 Adipocyte cell differentiation model: validated reference genes for qPCR gene expression analysis. PLoS One. 2012;7(5):e37517. doi: 10.1371/journal.pone.0037517
  • Ferguson BS, Nam H, Hopkins RG, et al. Impact of reference gene selection for target gene normalization on experimental outcome using real-time qRT-PCR in adipocytes. PLoS One. 2010;5(12):e15208. doi: 10.1371/journal.pone.0015208
  • Zhang J, Tang H, Zhang Y, et al. Identification of suitable reference genes for quantitative RT-PCR during 3T3-L1 adipocyte differentiation. Int J Mol Med. 2014;33(5):1209–1218. doi: 10.3892/ijmm.2014.1695
  • Asano H, Kanamori Y, Higurashi S, et al. Induction of beige-like adipocytes in 3T3-L1 cells. J Vet Med Sci. 2014;76(1):57–64. doi: 10.1292/jvms.13-0359
  • Voss G, Ceder Y. Two-tailed RT-qPCR for the quantification of A-to-I-edited microRNA isoforms. Curr Protoc. 2023;3(1):e645. doi: 10.1002/cpz1.645
  • Broeders S, Huber I, Grohmann L, et al. Guidelines for validation of qualitative real-time PCR methods. Trends Food Sci Technol. 2014;37(2):115–126. doi: 10.1016/j.tifs.2014.03.008
  • Taylor S, Wakem M, Dijkman G, et al. A practical approach to RT-qPCR-publishing data that conform to the MIQE guidelines. Methods. 2010;50(4):S1–S5. doi: 10.1016/j.ymeth.2010.01.005
  • Muñoz JJ, Anauate AC, Amaral AG, et al. Ppia is the most stable housekeeping gene for qRT-PCR normalization in kidneys of three Pkd1-deficient mouse models. Sci Rep. 2021;11(1):19798. doi: 10.1038/s41598-021-99366-x
  • Zeigerer A, Rodeheffer MS, McGraw TE, et al. Insulin regulates leptin secretion from 3T3-L1 adipocytes by a PI 3 kinase independent mechanism. Exp Cell Res. 2008;314(11–12):2249–2256. doi: 10.1016/j.yexcr.2008.04.003
  • Kanda T, Brown JD, Orasanu G, et al. PPARγ in the endothelium regulates metabolic responses to high-fat diet in mice. J Clin Invest. 2009;119(1):110–124. doi: 10.1172/JCI36233
  • Li J, Gong L, Liu S, et al. Adipose HuR protects against diet-induced obesity and insulin resistance. Nat Commun. 2019;10(1):2375. doi: 10.1038/s41467-019-10348-0
  • Babicki S, Arndt D, Marcu A, et al. Heatmapper: web-enabled heat mapping for all. Nucleic Acids Res. 2016;44(W1):W147–W153. doi: 10.1093/nar/gkw419
  • Vandesompele J, De Preter K, Pattyn F, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002;3(7):research0034.1. doi: 10.1186/gb-2002-3-7-research0034
  • Andersen CL, Jensen JL, Ørntoft TF. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004;64(15):5245–5250. doi: 10.1158/0008-5472.CAN-04-0496
  • Pfaffl MW, Tichopad A, Prgomet C, et al. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations. Biotechnol Lett. 2004;26(6):509–515. doi: 10.1023/B:BILE.0000019559.84305.47
  • Xie F, Xiao P, Chen D, et al. miRdeepfinder: a miRNA analysis tool for deep sequencing of plant small RNAs. Plant Mol Biol. 2012;80(1):75–84. doi: 10.1007/s11103-012-9885-2
  • Silver N, Best S, Jiang J, et al. Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol Biol. 2006;7(1):33. doi: 10.1186/1471-2199-7-33
  • Madsen MS, Siersbæk R, Boergesen M, et al. Peroxisome proliferator-activated receptor γ and C/EBPα synergistically activate key metabolic adipocyte genes by assisted loading. Mol Cell Biol. 2014;34(6):939–954. doi: 10.1128/MCB.01344-13
  • Thellin O, Zorzi W, Lakaye B, et al. Housekeeping genes as internal standards: use and limits. J Biotechnol. 1999;75(2–3):291–295. doi: 10.1016/S0168-1656(99)00163-7
  • Zhang Y, Li D, Sun B, et al. Do housekeeping genes exist? PLoS One. 2015;10(5):e0123691. doi: 10.1371/journal.pone.0123691
  • Adachi H, Tominaga H, Maruyama Y, et al. Stage-specific reference genes significant for quantitative PCR during mouse retinal development. Genes Cells. 2015;20(8):625–635. doi: 10.1111/gtc.12254
  • Svingen T, Letting H, Hadrup N, et al. Selection of reference genes for quantitative RT-PCR (RT-qPCR) analysis of rat tissues under physiological and toxicological conditions. PeerJ. 2015;3:e855. doi:10.7717/peerj.855
  • Alexander M, Curtis G, Avruch J, et al. Insulin regulation of protein biosynthesis in differentiated 3T3 adipocytes. Regulation of glyceraldehyde-3-phosphate dehydrogenase. J Biol Chem. 1985;260(22):11978–11985. doi: 10.1016/S0021-9258(17)38973-1
  • Alexander MC, Lomanto M, Nasrin N, et al. Insulin stimulates glyceraldehyde-3-phosphate dehydrogenase gene expression through cis-acting DNA sequences. Proc Natl Acad Sci, USA. 1988;85(14):5092–5096. doi: 10.1073/pnas.85.14.5092
  • Barroso I, Benito B, Garci-Jimenez C, et al. Norepinephrine, tri-iodothyronine and insulin upregulate glyceraldehyde-3-phosphate dehydrogenase mRNA during brown adipocyte differentiation. Eur J Endocrinol. 1999;141(2):169–179. doi: 10.1530/eje.0.1410169
  • Mohammadi-Farani A, Ghazi-Khansari M, Sahebgharani M. Glucose concentration in culture medium affects mRNA expression of TRPV1 and CB1 receptors and changes capsaicin toxicity in PC12 cells. Iran J Basic Med Sci. 2014;17(9):673–678.
  • Nishitani K, Hayakawa K, Tanaka S. Extracellular glucose levels in cultures of undifferentiated mouse trophoblast stem cells affect gene expression during subsequent differentiation with replicable cell line-dependent variation. J Reprod Dev. 2019;65(1):19–27. doi: 10.1262/jrd.2018-083
  • Bieback K, VA-T H, Hecker A, et al. Altered gene expression in human adipose stem cells cultured with fetal bovine serum compared to human supplements. Tissue Eng A. 2010;16(11):3467–3484. doi: 10.1089/ten.tea.2009.0727
  • Kwon D, Kim J-S, Cha B-H, et al. The effect of fetal bovine serum (FBS) on efficacy of cellular reprogramming for induced pluripotent stem cell (iPSC) generation. Cell Transplant. 2016;25(6):1025–1042. doi: 10.3727/096368915X689703
  • Lemma S, Avnet S, Meade MJ, et al. Validation of suitable housekeeping genes for the normalization of mRNA expression for studying tumor acidosis. Int J Mol Sci. 2018;19(10):2930. doi: 10.3390/ijms19102930
  • Rauschner M, Lange L, Hüsing T, et al. Impact of the acidic environment on gene expression and functional parameters of tumors in vitro and in vivo. J Exp Clin Cancer Res. 2021;40(1):10. doi: 10.1186/s13046-020-01815-4
  • Vergara M, Becerra S, Berrios J, et al. Differential effect of culture temperature and specific growth rate on CHO cell behavior in chemostat culture. PLoS One. 2014;9(4):e93865. doi: 10.1371/journal.pone.0093865
  • Torres M, Zúñiga R, Gutierrez M, et al. Mild hypothermia upregulates myc and xbp1s expression and improves anti-TNFα production in CHO cells. PLoS One. 2018;13(3):e0194510. doi: 10.1371/journal.pone.0194510
  • Chang-Liu CM, Woloschak GE. Effect of passage number on cellular response to DNA-damaging agents: cell survival and gene expression. Cancer Lett. 1997;113(1–2):77–86. doi: 10.1016/S0304-3835(97)04599-0
  • O’Driscoll L, Gammell P, McKiernan E, et al. Phenotypic and global gene expression profile changes between low passage and high passage MIN-6 cells. J Endocrinol. 2006;191(3):665–676. doi: 10.1677/joe.1.06894
  • Yang Q, Doshi U, Li N, et al. Effects of culture duration on gene expression of P450 isoforms, uptake and efflux transporters in primary hepatocytes cultured in the absence and presence of interleukin-6: implications for experimental design for the evaluation of downregulatory effects of biotherapeutics. Curr Drug Metab. 2012;13(7):938–946. doi: 10.2174/138920012802138570
  • Emam M, Thompson-Crispi K, Mallard B. The effect of immunological status, in-vitro treatment and culture time on expression of eleven candidate reference genes in bovine blood mononuclear cells. BMC Immunol. 2015;16(1):33. doi: 10.1186/s12865-015-0099-7
  • Mukhitov N, Adablah JE, Roper MG. Gene expression patterns in synchronized islet populations. Islets. 2019;11(2):21–32. doi: 10.1080/19382014.2019.1581544
  • Choi BH, Ahn IS, Kim YH, et al. Berberine reduces the expression of adipogenic enzymes and inflammatory molecules of 3T3-L1 adipocyte. Exp Mol Med. 2006;38(6):599–605. doi: 10.1038/emm.2006.71
  • Schreiber I, Dörpholz G, Ott CE, et al. BMPs as new insulin sensitizers: enhanced glucose uptake in mature 3T3-L1 adipocytes via PPARγ and GLUT4 upregulation. Sci Rep. 2017;7(1):17192. doi: 10.1038/s41598-017-17595-5
  • Brzeszczyńska J, Brzeszczyński F, Samuel K, et al. Validation of reference genes for gene expression studies by RT-qPCR in HepaRG cells during toxicity testing and disease modelling. Cells. 2020;9(3):770. doi: 10.3390/cells9030770