Anti-Convulsant Activity of Soxhlet Leaf Extracts of Ajuga Integrifolia Buch.-Ham. Ex D.Don (Lamiaceae) in Mice

References

• Thijs RD, Surges R, O’brien TJ, Sander JW. Epilepsy in adults. Lancet. 2019;393(10172):689–701.
   PubMed Web of Science ®Google Scholar
• Scharfman HE. The neurobiology of epilepsy. Curr Neurol Neurosci Rep. 2007;7(4):348–354.
   PubMed Web of Science ®Google Scholar
• Fisher RS, Acevedo C. official report: a practical clinical definition of epilepsy. Epilepsia. 2014;55(4):475–482.
   PubMed Web of Science ®Google Scholar
• Fisher RS, Cross JH, French JA, et al. Operational classification of seizure types by the International League Against Epilepsy: position Paper of the ILAE Commission for Classification and Terminology. Epilepsia. 2017;58(4):522–530.
   PubMed Web of Science ®Google Scholar
• Mosewich RK, So EL. A clinical approach to the classification of seizures and epileptic syndromes. Mayo Clin Proc. 1996;71(4):404–414.
   Web of Science ®Google Scholar
• Topçu G, Kökdil G, Türkmen Z, Voelter W, Adou E, Kingston DG. A new clerodane diterpene and other constituents from Ajuga chamaepitys ssp. laevigata. J Med. 2004;59B:584–588
   Google Scholar
• Pal A, Toppo FA, Chaurasiya PK, Singour PK, Pawar RS. In-vitro cytotoxicity study of methanolic fraction from Ajuga Bracteosa wall ex. benth on MCF-7 breast adenocarcinoma and hep-2 larynx carcinoma cell lines. Res j Pharmacogn. 2014;6(1):1–6.
   Google Scholar
• Kayani WK, Dilshad E, Ahmed T, Ismail H, Mirza B. Evaluation of Ajuga bracteosa for antioxidant, anti-inflammatory, analgesic, antidepressant and anticoagulant activities. BMC Complement Altern Med. 2016;16(1):1–13.
   PubMedGoogle Scholar
• Gautam R, Jachak SM, Saklani A. Anti-inflammatory effect of Ajuga bracteosa Wall Ex Benth. mediated through cyclooxygenase (COX) inhibition. J Ethnopharmacol. 2011;133(2):928–930.
   PubMed Web of Science ®Google Scholar
• Boudjelal A, Siracusa L, Henchiri C, et al. Antidiabetic effects of aqueous infusions of Artemisia herba-alba and Ajuga iva in alloxan-induced diabetic rats. Planta Med. 2015;81(9):696–704.
   PubMed Web of Science ®Google Scholar
• EL-Hilaly J, Amarouch M-Y, Morel N, Lyoussi B, Quetin-Leclercq J. Ajuga iva water extract antihypertensive effect on stroke-prone spontaneously hypertensive rats, vasorelaxant effects ex vivo and in vitro activity of fractions. J Ethnopharmacol. 2021;270(3):113791.
   PubMedGoogle Scholar
• Tahraoui A, El-Hilaly J, Israili Z, Lyoussi B. Ethnopharmacological survey of plants used in the traditional treatment of hypertension and diabetes in south-eastern Morocco (Errachidia province). J Ethnopharmacol. 2007;110(1):105–117.
   PubMed Web of Science ®Google Scholar
• Movahhedin N. Ajuga chamaecistus subsp. scoparia (Boiss.) Rech. f.: a new source of phytochemicals for antidiabetic, skin-care, and neuroprotective uses. Ind Crops and Prod. 2016;94:89–96.
   Web of Science ®Google Scholar
• Khalil EA, Afifi FU, Al-Hussaini M. Evaluation of the wound healing effect of some Jordanian traditional medicinal plants formulated in Pluronic F127 using mice (Mus musculus). J Ethnopharmacol. 2007;109(1):104–112.
   PubMed Web of Science ®Google Scholar
• Khanavi M, Davoodipoor AM, Sadati SN, Ardekani MRS, Sharifzadeh M. Antinociceptive effect of some extracts from Ajuga chamaecistus Ging. ssp. tomentella (Boiss.) Rech. f. aerial parts. DARU J Pharm Sci. 2014;22:1–6.
   Web of Science ®Google Scholar
• Rahman N, Ahmad M, Riaz M, Mehjabeen JN, Ahmad R. Phytochemical, antimicrobial, insecticidal and brine shrimp lethality bioassay of the crude methanolic extract of Ajuga parviflora Benth. Pak J Pharm Sci. 2013;26(4):751–756.
   PubMed Web of Science ®Google Scholar
• Setif A. Antibacterial activity of extract of Ajuga iva and Teucrium polium. Adv Environ Biol. 2011;5(2):491–495.
   Google Scholar
• Agarwal G, Pant A, Hore S. In vitro evaluation of anthelmintic efficacy of Trichilia and Ajuga species on Ascaridia galli. Hygeia J D Med. 2012;2(2):43–53.
   Google Scholar
• Rahman IU, Ijaz F, Iqbal Z, Afzal A. A novel survey of the ethno medicinal knowledge of dental problems in Manoor Valley (Northern Himalaya), Pakistan. J Ethnopharmacol. 2016;194:877–894.
   PubMed Web of Science ®Google Scholar
• Tafesse TB, Hymete A, Mekonnen Y, Tadesse M. Antidiabetic activity and phytochemical screening of extracts of the leaves of Ajuga remota Benth on alloxan-induced diabetic mice. BMC Complement Altern Med. 2017;17(1):1–9.
   PubMedGoogle Scholar
• Seifu A. Bioprospecting Potential of Ajuga Integrifolia for Access and Benefit Sharing. Rome, Italy: FAO; 2017.
   Google Scholar
• Kefalew A, Asfaw Z, Kelbessa E. Ethnobotany of medicinal plants in Ada’a District, East Shewa Zone of Oromia regional state, Ethiopia. J Ethnobiol Ethnomed. 2015;11(15):1–28.
   PubMedGoogle Scholar
• Degu S, Berihun A, Muluye R. Medicinal plants that used as repellent, insecticide and larvicide in Ethiopia. J.Pharm.Pharmacol. 2020;8(5):274–283.
   Google Scholar
• Tefera BN, Kim Y-D. Ethnobotanical study of medicinal plants in the Hawassa Zuria District, Sidama zone, Southern Ethiopia. J Ethnobiol Ethnomed. 2019;15(1):1–21.
   PubMedGoogle Scholar
• Teka A, Asfaw Z, Demissew S, Van Damme P. Medicinal plant use practice in four ethnic communities (Gurage, Mareqo, Qebena, and Silti), south central Ethiopia. J Ethnobiol Ethnome. 2020a;16(1):1–12.
   PubMed Web of Science ®Google Scholar
• Singh KJ, Thakur AK. Medicinal plants of the Shimla hills, Himachal Pradesh: a survey. J Herb Med. 2014;2(2):118–127.
   Google Scholar
• Wangpan T, Chetry LB, Tsering J, Tapi T, Tangjang S. Anti-Malarial Plants of Jonai, India: an Ethnobotanical Approach. Not Sci Biol. 2016;8(1):27–32.
   Google Scholar
• Keter LK, Mutiso PC. Ethnobotanical studies of medicinal plants used by Traditional Health Practitioners in the management of diabetes in Lower Eastern Province, Kenya. J Ethnopharmacol. 2012;139(1):74–80.
   PubMed Web of Science ®Google Scholar
• Njoroge GN, Bussmann RW. Diversity and utilization of antimalarial ethnophytotherapeutic remedies among the Kikuyus (Central Kenya). J Ethnobiol Ethnomed. 2006;2(1):1–7.
   PubMedGoogle Scholar
• Meresa A, Gemechu W, Basha H, et al. Herbal medicines for the management of diabetic mellitus in Ethiopia and Eretria including their phytochemical constituents. Am J Adv Drug Deliv. 2017;5(1):40–58.
   Google Scholar
• Giday M, Asfaw Z, Woldu Z. Medicinal plants of the Meinit ethnic group of Ethiopia: an ethnobotanical study. J Ethnopharmacol. 2009;124(3):513–521.
   PubMed Web of Science ®Google Scholar
• Asnake S, Teklehaymanot T, Hymete A, Erko B, Giday M. Survey of medicinal plants used to treat malaria by Sidama People of Boricha District, Sidama Zone, South Region of Ethiopia. Evid Based Complementary Altern Med. 2016;2016(1):1–9.
   Google Scholar
• Gedif T, Hahn H-J. The use of medicinal plants in self-care in rural central Ethiopia. J Ethnopharmacol. 2003;87(2–3):155–161.
   PubMed Web of Science ®Google Scholar
• Regassa R. Assessment of indigenous knowledge of medicinal plant practice and mode of service delivery in Hawassa city, southern Ethiopia. J Med Plants Res. 2013;7(9):517–535.
   Google Scholar
• Tilahun Y. Ethnobotanical study of traditional medicinal plants used in and around Adigrat town, eastern Tigray, Ethiopia. JMPS. 2018;6(4):11–19.
   Google Scholar
• Giday M, Asfaw Z, Woldu Z. Ethnomedicinal study of plants used by Sheko ethnic group of Ethiopia. J Ethnopharmacol. 2010;132(1):75–85.
   PubMed Web of Science ®Google Scholar
• Tesfaye S, Belete A, Engidawork E, Gedif T, Asres K. Ethnobotanical study of medicinal plants used by traditional healers to treat cancer-like symptoms in eleven districts, Ethiopia. Evid Based Complementary Altern Med. 2020;1(6):1–23.
   Google Scholar
• Parvez N, Yadav S. Ethnopharmacology of single herbal preparations of medicinal plants in Asendabo district, Jimma, Ethiopia. Indian J Tradit Knowl. 2010;9(4):724–729.
   Web of Science ®Google Scholar
• Teka A, Asfaw Z, Demissew S, Van Damme P. Traditional uses of medicinal plants practiced by the indigenous communities in Gurage Zone, south central Ethiopia. Ethnobot Res Appl. 2020b;19(2020):1–31.
   Google Scholar
• Mesfin F, Seta T, Assefa A. An ethnobotanical study of medicinal plants in Amaro Woreda, Ethiopia. Ethnobot Res Appl. 2014;12(2014):341–354.
   Google Scholar
• Getahun A. Some common medicinal and poisonous plants used in Ethiopian folk medicine. 1997.
   Google Scholar
• Teshome D. Concomitant use of medicinal plants and conventional medicines among hypertensive patients in five hospitals in Ethiopia. Ethiop J Health Dev. 2019;33(4):241–249.
   Web of Science ®Google Scholar
• Regassa R, Bekele T, Megersa M. Ethnobotanical study of traditional medicinal plants used to treat human ailments by Halaba people, southern Ethiopia. JMPS. 2017;5(4):36–47.
   Google Scholar
• Abera B. Medicinal plants used in traditional medicine by Oromo people, Ghimbi District, Southwest Ethiopia. J Ethnobiol Ethnomed. 2014;10(1):1–15.
   PubMedGoogle Scholar
• Atnafu H, Awas T, Alemu S, Wube S. Ethnobotanical study of medicinal plants in selale mountain ridges, North Shoa, Ethiopia. IJBC. 2018;2(6):567–577.
   Google Scholar
• Hailu W, Engidawork E. Evaluation of the diuretic activity of the aqueous and 80% methanol extracts of Ajuga remota Benth (Lamiaceae) leaves in mice. BMC Complement Altern Med. 2014;14(1):1–8.
   PubMedGoogle Scholar
• Nardos A, Makonnen E. In vivo antiplasmodial activity and toxicological assessment of hydroethanolic crude extract of Ajuga remota. Malar J. 2017;16(1):1–8.
   PubMedGoogle Scholar
• Asres K, Bucar F, Kartnig T, Witvrouw M, Pannecouque C. Antiviral activity against human immunodeficiency virus type 1 (HIV‐1) and type 2 (HIV‐2) of ethnobotanically selected Ethiopian medicinal plants. Phytotherapy Res. 2001;15(1):62–69
   PubMed Web of Science ®Google Scholar
• Cantrell CL, Rajab MS, Franzblau SG, Fronczek FR, Fischer NH. Antimycobacterial ergosterol-5, 8-endoperoxide from Ajuga remota. Planta Med. 1999;65(8):732–734.
   PubMed Web of Science ®Google Scholar
• Getaneh Y. Anti-Convulsant Activity of 80% Methanol Extract and Solvent Fractions of Ajuga Integrifolia Buch.- Ham(Lamiaceae) Leaves in Mice. Thesis. Addis Ababa University; 2020.
   Google Scholar
• Qasim S, Uttra AM, Hasan UH, Batool A. Evaluation of anticonvulsant potential of aqueous meth-anolic extract and various fractions of Ajuga bracteosa wall. J Appl Anim. 2017;2(2):137–146.
   Google Scholar
• Alene M. Evaluation of Antidiabetic Activity of Ajuga integrifolia (Lamiaceae) Root Extract and Solvent Fractions in Mice. Evid Based Complementary Altern Med. 2020;2020:1–11.
   Web of Science ®Google Scholar
• Tebeje BA. Phytochemical Screening of Secondary Metabolites of Extracts of the Plant Ajuga Integrifolia Leaves. chem.Mater. 2019;11(50):24281–24283.
   Google Scholar
• Vohra A, Kaur H. Chemical investigation of medicinal plant Ajuga bracteosa. J Nat Prod. 2011;1(1):37–45.
   Google Scholar
• Coll J, Tandrón Y. Isolation and identification of neo‐clerodane diterpenes from Ajuga remota by high‐performance liquid chromatography. Phytochem Anal. 2005;16(1):61–67.
   PubMed Web of Science ®Google Scholar
• Bekeri D, Adane L, Mamo F. Phytochemical Investigation and Isolation of Compounds From Ajuga integrifolia Root Extract. WJC. 2018;13(1):1–13.
   Google Scholar
• Liu W, Ge T, Pan Z, Leng Y, Lv J, Li B. The effects of herbal medicine on epilepsy. Oncotarget. 2017;8(29):48385.
   PubMedGoogle Scholar
• Council NR. Guide for the Care and Use of Laboratory Animals. 8 ed. National Academies Press; 2010.
   Google Scholar
• Salem GA, Alamyel FB, Abushaala FA, Hussain MS, Abusheba H, Sahu RP. Evaluation of the hepatoprotective, anti-inflammatory, antinociceptive and antiepileptic activities of Chrysanthemum trifurcatum. Biomed Pharmacother. 2019;117:1–7.
   Web of Science ®Google Scholar
• Raza M, Shaheen F, Choudhary M, et al. Anticonvulsant activities of ethanolic extract and aqueous fraction isolated from Delphinium denudatum. J Ethnopharmacol. 2001;78(1):73–78.
   PubMed Web of Science ®Google Scholar
• Mahendran S, Thippeswamy B, Veerapur V, Badami S. Anticonvulsant activity of embelin isolated from Embelia ribes. Phytomedicine. 2011;18(2–3):186–188.
   PubMed Web of Science ®Google Scholar
• Abdel-Zaher AO, Farghaly HS, Farrag MM, Abdel-Rahman MS, Abdel-Wahab BA. A potential mechanism for the ameliorative effect of thymoquinone on pentylenetetrazole-induced kindling and cognitive impairments in mice. Biomed Pharmacother. 2017;88:553–561.
   PubMed Web of Science ®Google Scholar
• Chang -C-C, Yang M-H, Wen H-M, Chern J-C. Estimation of total flavonoid content in propolis by two complementary colometric methods. J Food Drug Anal. 2002;10(3):178–182.
   Web of Science ®Google Scholar
• Maria R, Shirley M, Xavier C, et al. Preliminary phytochemical screening, total phenolic content and antibacterial activity of thirteen native species from Guayas province Ecuador. J King Saud Univ Sci. 2018;30(4):500–505.
   Web of Science ®Google Scholar
• Tabasum S, Khare S, Jain K. Spectrophotometric quantification of total phenolic, flavonoid, and alkaloid contents of Abrus precatorius L. seeds. Asian J Pharm Clin Res. 2016;9(2):371–374.
   Google Scholar
• Sayyah M, Mandgary A, Kamalinejad M. Evaluation of the anticonvulsant activity of the seed acetone extract of Ferula gummosa Boiss. against seizures induced by pentylenetetrazole and electroconvulsive shock in mice. J Ethnopharmacol. 2002;82(2–3):105–109.
   PubMed Web of Science ®Google Scholar
• Kuria KA, Chepkwony H, Govaerts C, et al. The Antiplasmodial Activity of Isolates from Ajuga r emota. J Nat Prod. 2002;65(5):789–793.
   PubMed Web of Science ®Google Scholar
• Akunal Turel C, Yunusoglu O. Oleanolic acid suppresses pentylenetetrazole-induced seizure in vivo. Int J Environ Health Res. 2023;33(5):529–540.
   PubMed Web of Science ®Google Scholar
• Aliyu MM, Musa AII, Kamal MJA, Mohammed MG. Phytochemical screening and anticonvulsant studies of ethyl acetate fraction of Globimetula braunii on laboratory animals. Asian Pac J Trop Biomed. 2014;4(4):285–289.
   PubMedGoogle Scholar
• Citraro R, Navarra M, Leo A. The anticonvulsant activity of a flavonoid-rich extract from Orange juice involves both NMDA and GABA-benzodiazepine receptor complexes. Molecules. 2016;21(19):1261.
   PubMedGoogle Scholar
• Samokhina E, Samokhin A. Neuropathological profile of the pentylenetetrazol (PTZ) kindling model. Int J Neurosci. 2018;128(11):1086–1096.
   PubMed Web of Science ®Google Scholar
• Dessalegn T. Anticonvulsant Activity of Soxhlet Leaf Extracts of Ajuga Integrifolia Buch.Ham Ex D. Don (Lamiaceae) in Mice. Thesis. Addis Ababa University; 2021.
   Google Scholar