Publication Cover
Inhalation Toxicology
International Forum for Respiratory Research
Volume 7, 1995 - Issue 2
10
Views
12
CrossRef citations to date
0
Altmetric
Research Article

Incorporation of Bromodeoxyuridine as a Method to Quantify Cell Proliferation in Bronchiolar-Alveolar Duct Regions of Asbestos-Exposed Mice

&
Pages 215-224 | Received 03 Apr 1994, Accepted 20 Jun 1994, Published online: 20 Oct 2008

References

  • Bauman M. D., Jetten A. M., Bonner J. C., Kumar R. K., Bennett R. A., Brody A. R. Secretion of a platelet-derived growth factor by rat alveolar macrophages exposed to particulates in vitro. Eur. J. Cell Biol. 1990; 51: 327–334
  • Bonner J. C., Osornio-Vargas A. R., Badgett A., Brody A. R. Differential proliferation of rat lung fibroblasts induced by the platelet-derived growth factor-AA, -AB, and -BB isoforms secreted by rat alveolar macrophages. Am. J. Respir. Cell. Mol. Biol. 1991; 5: 539–547
  • Bonner J. C., Coodell A. L., Brody A. R. Chrysotile asbestos up-regulates gene expression and production of α-receptors for platelet-derived growth factor (PDCF-AA) on rat lung fibroblasts. J. Clin. Invest. 1993; 92: 425–430
  • Brody A. R. Asbestos-induced lung disease. Environ. Health Perspect. 1993; 100: 21–30
  • Brody A. R., Overby L. H. Incorporation of tritiated thymidine by epithelial and interstitial cells in bronchiolar-alveolar regions of asbestos-exposed rats. Am. J. Pathol. 1989; 134: 133–140
  • Brody A. R., Hill L. H., Adkins B., O'Conner R. W. Chrysotile asbestos inhalation in rats: Deposition pattern and reaction of alveolar epithelium and pulmonary macrophages. Am. Rev. Respir. Dis. 1981; 123: 670–679
  • Chang L. Y., Overby L. H., Brody A. R., Crapo J. D. Progressive lung cell reactions and extracellular matrix production after a brief exposure to asbestos. Am. I. Pathol. 1988; 131: 156–170
  • Coin P. C., Roggli V., Brody A. R. Pulmonary fibrogenesis and BrdU incorporation after three consecutive inhalation exposures to chrysotile asbestos. Am. Rev. Respir. Dis. 1992; 145: A328
  • Dean P. N., Dolbeare F., Cratzner H., Rice C. G., Gray J. W. Cell-cycle analysis using a monoclonal antibody to BrdUrd. Cell Tissue Kinet. 1984; 17: 427–436
  • Eldridge S. R., Tilbury L. F., Goldsworthy T. L., Butterworth B. E. Measurement of chemically induced cell proliferation in rodent liver and kidney: A comparison of 5-bromo-2′-deoxyuridine and [3H]thymidine administered by injection or osmotic pump. Carcinogenesis 1990; 11: 2245–2251
  • Gratzner H. G. Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science 1982; 218: 474–475
  • Hamada S. A double labeling technique combining 3H-thymidine autoradiography with BrdUrd immunocytochemistry. Acta Histochem. Cytochem. 1985; 18: 267–270
  • Kalter V. G., Brody A. R. Receptors for TGFβ on rat lung fibroblasts have higher affinity for TGFβ1 than β2. Am. J. Respir. Cell. Mol. Biol. 1990; 4: 397–407
  • Kumar R. K., Bennett R. A., Brody A. R. A homologue of platelet-derived growth factor produced by rat alveolar macrophages. FASEB J. 1988; 2: 2272–2277
  • Lanier T. L., Berger E. K., Eacho P. I. Comparison of 5-bromo-2-deoxyuridine and (3H]thymidine for studies of hepatocellular proliferation in rodents. Carcinogenesis 1989; 10: 1341–1343
  • Lasky J. A., Coin P. G., Lindroos P. M., Ostrowski L. E., Brody A. R., Bonner J. C. Chrysotile asbestos stimulates gene expression and secretion of PDGF-AA by rat lung fibroblasts in vitro: Evidence for an autocrine loop. Am. J. Respir. Cell. Mol. Biol. 1994, in press.
  • McGavran P. D., Butterick C. J., Brody A. R. Tritiated thymidine incorporation and the development of an interstitial lesion in the bronchiolar-alveolar regions of the lungs of normal and complement deficient mice after inhalation of chrysotile asbestos. J. Environ. Pathol. Toxicol. Oncol. 1990a; 9: 377–392
  • McCavran P. D., Moore L. B., Brody A. R. Inhalation of chrysotile asbestos induces rapid cellular proliferation in small pulmonary vessels of mice and rats. Am. I. Pathol. 1990b; 136: 695–705
  • McLean I. W., Nakane P. K. Periodate-lysine-paraformaldehyde fixative: A new fixative for immunoelectron microscopy. J. Histochem. Cytochem. 1974; 22: 1077–1083
  • Montuenga L. M., Springall D. R., Gaer J., Winter R. J. D., Zhao L., McBride J. T., Taylor K. M., Barer C., Polak J. M. CCRP-immunoreactive endocrine cell proliferation in normal and hypoxic rat lung studied by immunocytochemical detection of incorporation of 5′-bromode- oxyuridine. Cell Tissue Res. 1992; 268: 9–15
  • Morstyn G., Hsu S. M., Kinsella T., Cratzner H., Russo A., Mitchell J. B. Bromodeoxy-uridine in tumors and chromosomes detected with a monoclonal antibody. J. Clin. Invest. 1983; 72: 1844–1850
  • Pinkerton K. E., Pratt P. C., Brody A. R., Crapo J. D. Fiber localization and its relationship to lung reactions in rats after chronic exposure to chrysotile asbestos. Am. J. Pathol. 1984; 117: 484–493
  • Raza A., Preisler H. D., Mayers G. L., Bankert R. Rapid enumeration of S-phase cells by means of monoclonal antibodies. N. Engl. j. Med. 1984; 310: 991
  • Raza A., Ucar K., Bhayana R., Kempski M., Preisler H. D. Utility and sensitivity of anti BrdU antibodies in assessing S-phase cells compared to autoradiography. Cell. Biochem. Fund. 1985; 3: 149–153
  • Ross R., Raines E. W., Bowen-Pope D. F. The biology of platelet-derived growth factor. Cell 1986; 46: 155–169
  • Selikoff I. J., Lee D. H. Asbestos and disease. Academic Press., New York 1978
  • Silvestrini R., Costa A., Veneroni S., Del Bino G., Persici P. Comparative analysis of different approaches to investigate cell kinetics. Cell Tissue Kinet. 1988; 21: 123–131
  • Snedecor G. W., Cochran W. G. Statistical methods. Iowa State University Press., Ames 1980
  • Sporn M. B., Roberts A. B., Wakefield L. M., Assoian R. K. Transforming growth factor: Biological function and chemical structure. Science 1986; 233: 532–534
  • Wagner J. C., Berry G., Skidmore J. W., Timbrell V. The effects of the inhalation of asbestos in rats. Br. J. Cancer 1974; 29: 252–269
  • Warheit D. B., Chang L. Y., Hill L. H., Hook G. E. R., Crapo J. D., Brody A. R. Pulmonary macrophage accumulation and asbestos-induced lesions at sites of fiber deposition. Am. Rev. Respir. Dis. 1984; 129: 301–310

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.