True volumetric method for flow cytometric enumeration of CD34 ⁺ stem cells and its agreement with a standard bead-based single-platform protocol

References

• Sutherland DR, Nayyar R, Acton E, Giftakis A, Dean S, Mosiman VL. Comparison of two single-platform ISHAGE-based CD34 enumeration protocols on BD FACSCalibur and FACSCanto flow cytometers. Cytotherapy. 2009;11: 595–605.
   PubMed Web of Science ®Google Scholar
• Keeney M, Gratama JW, Sutherland DR. Critical role of flow cytometry in evaluating peripheral blood hematopoietic stem cell grafts. Cytometry A. 2004;58:72–5.
   PubMedGoogle Scholar
• Gratama JW, Braakman E, Kraan J, Lankheet P, Levering WH, Van Den Beemd MW, . Comparison of single and dual-platform assay formats for CD34 + haematopoietic progenitor cell enumeration. Clin Lab Haematol. 1999;21: 337–46.
   PubMedGoogle Scholar
• Menendez P, Redondo O, Rodriguez A, Lopez-Berges MC, Ercilla G, Lopez A, . Comparison between a lyse-and-then-wash method and a lyse-non-wash technique for the enumeration of CD34 + hematopoietic progenitor cells. Cytometry. 1998;34:264–71.
   PubMedGoogle Scholar
• Keeney M, Sutherland DR. Stem cell enumeration by flow cytometry: current concepts and recent developments in CD34 + cell enumeration. Cytotherapy. 2000;2:395–402.
   PubMed Web of Science ®Google Scholar
• Chin-Yee I, Anderson L, Keeney M, Sutherland DR. Quality assurance of stem cell enumeration by flow cytometry. Canadian QASE Study Group. Cytometry. 1997;30:296–303.
   PubMedGoogle Scholar
• Oelschlaegel U, Bornhaeuser M, Thiede C, Ehninger G, Hoelig K. HPC enumeration with the Sysmex XE-2100 can guide further flow cytometric CD34(+) measurements and timing of leukaphereses. Cytotherapy. 2003;5:414–9.
   PubMed Web of Science ®Google Scholar
• Iscove NN, Senn JS, Till JE, McCulloch EA. Colony formation by normal and leukemic human marrow cells in culture: effect of conditioned medium from human leukocytes. Blood. 1971;37:1–5.
   PubMed Web of Science ®Google Scholar
• Venditti A, Battaglia A, Del Poeta G, Buccisano F, Maurillo L, Tamburini A, . Enumeration of CD34 + hematopoietic progenitor cells for clinical transplantation: comparison of three different methods. Bone Marrow Transplant. 1999; 24:1019–27.
   PubMed Web of Science ®Google Scholar
• Leuner S, Arland M, Kahl C, Jentsch-Ullrich K, Franke A, Hoffkes HG. Enumeration of CD34-positive hematopoietic progenitor cells by flow cytometry: comparison of a volumetric assay and the ISHAGE gating strategy. Bone Marrow Transplant. 1998;22:699–706.
   PubMed Web of Science ®Google Scholar
• Gratama JW, Orfao A, Barnett D, Brando B, Huber A, Janossy G, . Flow cytometric enumeration of CD34 + hematopoietic stem and progenitor cells. European Working Group on Clinical Cell Analysis. Cytometry. 1998;34:128–42.
   PubMedGoogle Scholar
• Guttridge MG, Belfield H, Hollyman D, Lankester A, Watt SM. An internal positive control for the enumeration of CD45(+) and CD34(+) cells by flow cytometry allows monitoring of reagent and operator performance. Cytotherapy. 2007;9:275–82.
   PubMed Web of Science ®Google Scholar
• Sutherland DR, Anderson L, Keeney M, Nayar R, Chin-Yee I. The ISHAGE guidelines for CD34 + cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering. J Hematother. 1996;5:213–26.
   PubMedGoogle Scholar
• Keeney M, Chin-Yee I, Weir K, Popma J, Nayar R, Sutherland DR. Single platform flow cytometric absolute CD34 + cell counts based on the ISHAGE guidelines. International Society of Hematotherapy and Graft Engineering. Cytometry. 1998;34:61–70.
   PubMedGoogle Scholar
• Brocklebank AM, Sparrow RL. Enumeration of CD34 + cells in cord blood: a variation on a single-platform flow cytometric method based on the ISHAGE gating strategy. Cytometry. 2001;46:254–61.
   PubMedGoogle Scholar
• Barbosa IL, Sousa ME, Godinho MI, Sousa F, Carvalhais A. Single- versus dual-platform assays for human CD34 + cell enumeration. Cytometry. 1999;38:274–9.
   PubMedGoogle Scholar
• Hubl W, Iturraspe J, Martinez GA, Hutcheson CE, Roberts CG, Fisk DD, . Measurement of absolute concentration and viability of CD34 + cells in cord blood and cord blood products using fluorescent beads and cyanine nucleic acid dyes. Cytometry. 1998;34:121–7.
   PubMedGoogle Scholar
• Gratama JW, Kraan J, Keeney M, Sutherland DR, Granger V, Barnett D. Validation of the single-platform ISHAGE method for CD34(+) hematopoietic stem and progenitor cell enumeration in an international multicenter study. Cytotherapy. 2003;5:55–65.
   PubMed Web of Science ®Google Scholar
• Gisselo CG, Roer O, Hoffmann MH, Hansen MB, Taaning E, Johnsen HE. Assessing agreement between CD34 enumeration by flow cytometry and volumetric analysis. Bone Marrow Transplant. 2002;29:699–703.
   PubMedGoogle Scholar
• Barnett D, Granger V, Whitby L, Storie I, Reilly JT. Absolute CD4 + T-lymphocyte and CD34 + stem cell counts by single-platform flow cytometry: the way forward. Br J Haematol. 1999;106:1059–62.
   PubMed Web of Science ®Google Scholar
• Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986;1:307–10.
   PubMed Web of Science ®Google Scholar
• Bland JM, Altman DG. Measuring agreement in method comparison studies. Stat Methods Med Res. 1999;8: 135–60.
   PubMed Web of Science ®Google Scholar
• Gajkowska A, Oldak T, Jastrzewska M, Machaj EK, Walewski J, Kraszewska E, . Flow cytometric enumeration of CD34 + hematopoietic stem and progenitor cells in leukapheresis product and bone marrow for clinical transplantation: a comparison of three methods. Folia Histochem Cytobiol. 2006;44:53–60.
   PubMed Web of Science ®Google Scholar
• Barnett D, Granger V, Storie I, Peel J, Pollitt R, Smart T, . Quality assessment of CD34 + stem cell enumeration: experience of the United Kingdom National External Quality Assessment Scheme (UK NEQAS) using a unique stable whole blood preparation. Br J Haematol. 1998;102:553–65.
   PubMed Web of Science ®Google Scholar
• Barnett D, Janossy G, Lubenko A, Matutes E, Newland A, Reilly JT. Guideline for the flow cytometric enumeration of CD34 + haematopoietic stem cells. Prepared by the CD34 + haematopoietic stem cell working party. General Haematology Task Force of the British Committee for Standards in Haematology. Clin Lab Haematol. 1999;21:301–8.
   PubMedGoogle Scholar
• Rivadeneyra-Espinoza L, Perez-Romano B, Gonzalez-Flores A, Guzman-Garcia MO, Carvajal-Armora F, Ruiz-Arguelles A. Instrument- and protocol-dependent variation in the enumeration of CD34 + cells by flow cytometry. Transfusion. 2006;46:530–6.
   PubMedGoogle Scholar
• Lemarie C, Bouchet G, Sielleur I, Durand V, Navarro F, Calmels B, . A new single-platform method for the enumeration of CD34 + cells. Cytotherapy. 2009;11:804–6.
   PubMed Web of Science ®Google Scholar
• Manasa J, Musabaike H, Masimirembwa C, Burke E, Luthy R, Mudzori J. Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe. Clin Vaccine Immunol. 2007;14:293–8.
   PubMed Web of Science ®Google Scholar
• Fryland M, Chaillet P, Zachariah R, Barnaba A, Bonte L, Andereassen R, . The Partec CyFlow Counter could provide an option for CD4 + T-cell monitoring in the context of scaling-up antiretroviral treatment at the district level in Malawi. Trans R Soc Trop Med Hyg. 2006; 100:980–5.
   PubMedGoogle Scholar
• Dieye TN, Vereecken C, Diallo AA, Ondoa P, Diaw PA, Camara M, . Absolute CD4 T-cell counting in resource-poor settings: direct volumetric measurements versus bead-based clinical flow cytometry instruments. J Acquir Immune Defic Syndr. 2005;39:32–7.
   PubMedGoogle Scholar
• Cassens U, Gohde W, Kuling G, Groning A, Schlenke P, Lehman LG, . Simplified volumetric flow cytometry allows feasible and accurate determination of CD4 T lymphocytes in immunodeficient patients worldwide. Antivir Ther. 2004;9:395–405.
   PubMedGoogle Scholar
• Bland JM, Altman DG. Measurement error. Br Med J. 1996;312:1654.
   PubMed Web of Science ®Google Scholar
• Brando B, Gohde W Jr, Scarpati B, D’Avanzo G. The ‘vanishing counting bead’ phenomenon: effect on absolute CD34 + cell counting in phosphate-buffered saline-diluted leukapheresis samples. Cytometry. 2001;43:154–60.
   PubMedGoogle Scholar
• Phillips WA, Hosking CS, Shelton MJ. Effect of ammonium chloride treatment on human polymorphonuclear leucocyte iodination. J Clin Pathol. 1983;36:808–10.
   PubMedGoogle Scholar
• Cassens U, Gutensohn K, Garritsen H, Kelsch R, Kuehnl P, Sibrowski W. The influence of different erythrocyte lysing procedures on flow cytometric determination of CD34 + cells in umbilical cord blood transplants. Transfus Med. 1998; 8:111–8.
   PubMedGoogle Scholar