In order to find polymorphic tissue enzymes and suitable buffer systems for use in population genetic studies of Norway pout ( Trisopterus esmarkii ), a pilot study was performed using 30 individual specimens from one location. A set of potentially useful genetic markers was established, based on electrophoretic analysis of 22 different tissue enzymes in three different buffer systems. The initial analyses revealed 31 putative loci, from which eight were considered to be reliable in routine scoring. Here, a total of 1797 specimens of Norway pout, from 23 individual locations in the fjords and coastal waters of Norway, were scored for these eight loci. Variant alleles were found at all loci. At least five of the loci were polymorphic by the 0.99 criterion ( LDH-2 *, GPI-1 *, GPI-2 *, G3PDH-2 *, IDHP-2 *) and one locus also by the 0.95 criterion ( LDH-2 *). The frequency of polymorphic loci were P 0.99 ≥ 0.625 and P 0.95 = 0.125. The average heterozygosity over all loci based on Hardy-Weinberg expectations was H = 0.072 (±0.050). Here the details of electrophoretic conditions, staining procedures and tissue manifestation of the loci and the allele designations are described.
Tissue enzyme loci for routine genotyping in Norway pout ( Trisopterus esmarkii Nilsson, 1855): Electrophoresis conditions, tissue manifestations, allele designations and basic population genetic parameters
Reprints and Corporate Permissions
Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?
To request a reprint or corporate permissions for this article, please click on the relevant link below:
Academic Permissions
Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?
Obtain permissions instantly via Rightslink by clicking on the button below:
If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.
Related research
People also read lists articles that other readers of this article have read.
Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.
Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.