Optimization of flow cytometry methods to differentiate epithelial cell origin

Ishana Somersa College of Science and Engineering, Flinders University, Bedford Park, Australia

Amy Brittona College of Science and Engineering, Flinders University, Bedford Park, Australia

Giles Bestb Flinders Health and Medical Research Institute, Flinders University Flow Cytometry Facility, Bedford Park, Australia

Duncan Taylora College of Science and Engineering, Flinders University, Bedford Park, Australia;c Forensic Science SA, Adelaide, Australia

https://orcid.org/0000-0003-0633-7424

Roland A.H. van Oorschotd Office of the Chief Forensic Scientist, Victoria Police Forensic Services Department, Macleod, Australia;e School of Agriculture, Biomedicine and Environment, La Trobe University, Bundoora, Australia

Mariya Goraya College of Science and Engineering, Flinders University, Bedford Park, AustraliaCorrespondence[email protected]

ABSTRACT

The ability to link a DNA profile to a source of biological material has significant implications in forensic investigations. Epithelial cells are the main constituent of many of the samples collected as part of forensic casework. However, it is currently not possible to distinguish between or isolate epithelial cells from different anatomical regions. Flow cytometry represents a potential method that could be used in this context. This study was conducted to optimize procedures for the collection and processing of epidermal, buccal, vaginal and penile epithelial cells prior to analysis by flow cytometry.

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Optimization of flow cytometry methods to differentiate epithelial cell origin

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Optimization of flow cytometry methods to differentiate epithelial cell origin

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