Mixture of enzyme-processed Panax ginseng and Gastrodia elata extract prevents UVB-induced decrease of procollagen type 1 and increase of MMP-1 and IL-6 in human dermal fibroblasts

ABSTRACT

According to the previously described anti-photoaging effect of the enzyme-processed Panax ginseng extract and Gastrodia elata extract, we hypothesized that the combination of the two extracts would have superior effect to protect human skin from UVB radiation. Besides, the mixture of active components isolated from herbal extracts, ginsenoside F2, and α-gastrodin was investigated on the photo-protective capability. The expression of aging-related markers including matrix metalloproteinase-1 (MMP-1), interleukin-6 (IL-6), and procollagen type 1 was evaluated using ELISA kits. It was reported that the herbal extract at a Panax ginseng extract to Gastrodia elata extract ratio of 1:10 (w/w) and the compound mixture with equal proportion of ginsenoside F2 and α-gastrodin exhibited significant inhibition of MMP-1 and IL-6 production, and marked upregulation of procollagen type 1 formation. Thus, the combination of either the enzyme-processed herbal extracts or their active components would enhance the properties of prevention and treatment of UVB-induced skin damage.

GRAPHICAL ABSTRACT

The reverse effect of EPG on the UVB-induced damage via inhibiting MMP-1 and IL-6 production and accelerating procollagen type 1 synthesis.

KEYWORDS:

• Panax ginseng
• Gastrodia elata
• ginsenoside F2
• α-gastrodin
• ultraviolet B (UVB) irradiation

Contribution of authors

The experiments were designed by H.T.T.N, E.H., and T-H.Y. The experiments were performed by H.T.T.N, S.a.S, and J-E.Y. The data were analyzed by H.T.T.N, E.H., Q.T.N.N, and N.Q.D. The manuscript was edited by H.T.T.N, Q.T.N.N, and N.Q.D. The experiment was supervised by T-H.Y.

Disclosure statement

The authors have no conflicts of interest to declare.