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ABSTRACT
The simple and rapid identification of meat via DNA-based methods is still subject to the conditions of complex and time-consuming preprocessing. It usually takes 3–4 h to obtain DNA sample using conventionally available DNA extraction kits. The existing approaches are cumbersome, require large equipment, and are not suitable for field operation. This study developed a swelling microneedle patch, constituted by micron-sized copolymers of methyl vinyl ether and maleic acid (PMVE/MA). The microneedle patch was applied to the meat sample for 1 min before being pulled out and rinsed with Tris-EDTA (TE) buffer to obtain DNA, which could be used for polymerase chain reaction (PCR) without purification. The DNA extracted by the microneedle patch could meet the needs for amplification and identification of meat samples. The developed approach which combines the patch-based extraction method and conventional PCR amplification is quick, simple, and reliable for DNA extraction and identification of meat samples.
Acknowledgements
The authors would like to thank all the reviewers who participated in the review, as well as the MJ Editor for providing English editing services during the preparation of this manuscript.
Associated content
Gel electrophoresis patterns of mutton DNA were identified by combining microneedle extraction with RPA; Primers were tested in this study.
Abbreviations used
PMVE/MA, copolymers of methyl vinyl ether and maleic acid; PVA, poly (vinyl alcohol); PCR, polymerase chain reaction; RPA, recombinase polymerase amplification; PEG, Polyethylene glycol; PDMS, polydimethylsiloxane; PBS, phosphate-buffered saline; qRT-PCR, quantitative real-time PCR; LFS, lateral flow strips.
Disclosure statement
No potential conflict of interest was reported by the author(s).