Genome-wide identification, evolutionary analysis of 4CL gene family and expression analysis in banana

ABSTRACT

4-coumaric acid coenzyme A ligase (4CL) plays an important role in plant growth, development and resistance. Based on the Musa acuminata (A genome) genome database, we performed genome-wide identification and evolutionary analysis of the 4CL gene family.The results showed that there were 20 members of 4CL, and the amino acids encoded ranged from 160 to 700, all of which were hydrophobic proteins. All the members of the 4CL gene family had no signal peptide, and phosphorylation sites ranged from 10 to 60. The 4CL genes were uniformly distributed and disordered in the chromosomes. All protein members can interact with each other.Transcriptome expression analysis showed that Ma4CL3-2 expression was down-regulated and Ma4CL11-1 expression was up-regulated. Musa acuminata genomics has 22 and 19 pairs of co-linear members with Musa balbisiana and Musa itinerans genomics, respectively.The above results provide a good foundation for further research on the function of the 4CL genes and resistance breeding.

Highlights

1. Twenty 4CL gene family members were found in banana (Musa acuminata) genomes.

2. Banana 4CLs were expressed in response to low temperature stress.

3. The members of the banana 4CL gene family were found to be relatively structurally diverse, and it was further hypothesised that 4CL may be involved in plant lignin synthesis and closely related to the process of plant differentiation into monocotyledons and dicotyledons, based on the hypothesis of Huang et al.

KEYWORDS:

• Musa acuminata
• 4CL
• genetic family identification
• function prediction
• evolutionary analysis

Acknowledgements

All authors contributed to the study’s conception and design. ZhongXiong Lai presented the thesis topics and gave valuable advice, MengGe Wang analysed the data, created the figures and wrote the paper, Liang Jia to make additions and revisions to the content of the paper, YanYing Liu and ChunYu Zhang gave useful advice and provided the language assistance. ZhongXiong Lai, YuJi Huang, YuKun Chen and YuLing Lin revised the paper. All authors read and approved the final manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Declaration of competing interests

The authors declare no conflict of competing interests.

Additional information

Funding

This work was funded by National Key Research and Development Program of China (2019YFD1000900) and Earmarked Fund for China Agriculture Research System (CARS-31-15).