Abstract
The excision repair cross-complementing group 2 (ERCC2) gene encodes a DNA repair protein, which is absolutely necessary in nucleotide excision repair. A polymorphism in codon 751 that induces a Lys-Gln substitution has been suggested to reduce the DNA repair capacity. Therefore, we conducted a matched case-control study to investigate the role ERCC2 Lys751Gln polymorphism in the development of lung cancer in the Chinese population. The genotype of ERCC2 gene was analyzed by di-allele-specific-amplification with artificially modified primers (diASA-AMP) in 200 original lung cancer cases and 200 controls. The results showed that carriers of Lys/Gln and Gln/Gln genotypes had a 3.32-fold higher risk of lung cancer compared with carriers of Lys/Lys genotype. Furthermore, the mutant genotype of 751 Gln allele was found to be associated with an increased risk in both lung squamous cell carcinoma and lung adenocarcinoma. However, no significant interaction between 751Gln variants and smoking was observed after stratifying according to the smoking status in this study. The results suggest that the Lys751Gln polymorphism in ERCC2 gene is a potential biomarker for susceptibility of lung cancer in the Chinese population.
*Supported by National Natural Science Foundation of China (Grant No. 30170791), Jiangsu Province Social Development Research Foundation (BS2000034), and Foundation of Graduate Creative Program of Jiangsu (XM 04-70)
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*Supported by National Natural Science Foundation of China (Grant No. 30170791), Jiangsu Province Social Development Research Foundation (BS2000034), and Foundation of Graduate Creative Program of Jiangsu (XM 04-70)