Probiotic BioPlus® PS modulate shrimp-tilapia polyculture pond soil microbiome and exhibit bioremediation potential

ABSTRACT

The study evaluated the effects of the probiotic BioPlus® PS on shrimp-tilapia polyculture pond soil using an integrated approach of physical-chemical analysis, metagenomics, and Pfeiffer circular chromatography. Soil was collected from a shrimp farm immediately after harvest and distributed in 75 plastic boxes with a useful volume of 1 L. The probiotic was applied once at the following doses in triplicate: 0, 150, 300, 1000 and 2000 g ha⁻¹. After fifteen days, we verified a significant reduction in the content of organic matter, oxidizable carbon and potential acidity. Metagenomics demonstrated that the probiotic increased α diversity, while decreasing the relative abundance of Vibrio parahaemolyticus. Pfeiffer chromatography showed that the two samples that received higher doses showed characteristics associated with higher quality soils and higher enzymatic activity. Our results suggest that the application of the probiotic BioPlus® PS in shrimp pond soils has bioremediation potential and can positively modulate the soil microbiome.

KEYWORDS:

• Metagenomics
• aquaculture
• Bacillus
• bioremediation
• probiotics

Acknowledgments

We would like to acknowledge the Coordination for the Improvement of Higher Education Personnel (CAPES) for a scholarship granted to H.M. de Oliveira and for partial financial support (Finance Code 001), the National Council for Scientific and Technological Development (CNPq) for financial support to J.L.P. Mouriño (CNPq 301524/2017-3) and M.L. Martins (CNPq 306635/2018-6), and the Foundation for the Support of University Research and Extension (FAPEU) for a scholarship to M. A. Martins.

Disclosure statement

The following two co-authors have potential competing interests: Benny Shapira: Global Research and Development Manager, Phibro Animal Health Corporation. Employed at the company that sells the product evaluated in the study. Ligia Aparecida de Godoi: Development and Application Coordinator at Christian Hansen. Employed at the company that developed the product evaluated in the study.

Data availability statement

The data that support the findings of this study are available from the corresponding author upon request.

Authors contribution

HMO: Experimental execution, Investigation, Methodology, Writing – Original Draft, Writing. MSO: Experimental execution, Writing, Review and Editing. MAM: Writing – Original Draft, Writing – Review & Editing, Formal Analysis. GRL: Conceptualization, Investigation, Methodology. MBF: Investigation, Methodology. GFAJ: Investigation, Methodology. LAG: Resources, Conceptualization. BS: Resources, Conceptualization. MLM: Methodology, Resources, Writing – Review & Editing, Funding Acquisition. JLPM: Methodology, Conceptualization, Resources, Funding Acquisition.

Additional information

Funding

Part of this study was funded by the Coordination for the Improvement of Higher Education Personnel (CAPES) for a scholarship granted to H.M. de Oliveira and for partial financial support (Finance Code 001).