Genome-wide identification of pseudo-response regulator (PRR) family members in cabbage (Brassica oleracea var. capitata L.) and their expression in response to abiotic stress

ABSTRACT

PRRs (pseudo-response regulators) genes play crucial roles in plant growth and development, stress response and other life activities. However, this gene family in cabbage (Brassica oleracea var. capitata L) has yet to be comprehensively studied. To fully understand the status of PRR in cabbage, ten BoPRR genes were extracted from the whole genome of cabbage through bioinformatics analysis, and their gene structure, protein conservative motifs, cis acting elements and collinearity relationships were identified and analysed. Quantitative real-time PCR (qRT-PCR) was used to explore the expression patterns of BoPRR genes in cabbage leaves under various abiotic stresses. Based on phylogenetic analysis, the ten BoPRR genes were divided into three branches, located on four chromosomes. In addition, many photoresponsive elements exist in the promoter region, and specific response elements with low temperature, drought and abscisic acid (ABA). In terms of gene expression patterns, all ten BoPRR genes were found to be expressed in cabbage leaves and showed response to abiotic stress. This study represents the first comprehensive examination of the PRR family in cabbage, providing a theoretical basis for further research on abiotic stress and offering a new gene resource for breeding stress-resistant varieties.

KEYWORDS:

• Abiotic stress
• BoPRR genes
• cabbage
• genome wide analysis

Acknowledgements

SJH secured the funding for the project. XYY and SJH conceived and designed the experiments.

XYY and JYJ performed the experiments. XYY wrote the paper. SJH and MAR revised the

manuscript. All authors read and approved the manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Data availability statement

All data generated or analysed in this study are included in the articles published here and referenced for supplementary information.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/14620316.2023.2263763

Additional information

Funding

This work was supported by the National Natural Science Foundation of China [No. 32272728, 31872108].