Genome-wide identification and characterisation of Aquaporins in Rosa chinensis

ABSTRACT

Aquaporins (AQPs) are small integral membrane proteins that facilitate the efficient transport of small solutes like water, metalloids, urea, and CO2 across the membrane. Plant aquaporins are known to be involved in vital physiological processes like seed germination, stomatal movement, cell elongation, and reproductive growth. In the present study, 36 AQPs were identified through genome-wide computational analysis performed in the rose genome. Subsequent phylogenetic analysis classified the AQPs into five subfamilies, including 10 Plasma membrane intrinsic proteins (PIPs), 8 Tonoplast intrinsic proteins (TIPs), 9 Nodulin intrinsic proteins (NIPs), 3 Small intrinsic proteins (SIPs), and 6 uncharacterised intrinsic proteins (XIPs). Based on the prediction of silicon solute specificity, one of the NIPs is predicted to be a silicon transporter. Structural analysis revealed the characteristic six trans-membrane domains and two-half alpha helix harbouring two conserved NPA motifs in the majority of the predicted AQPs. The protein tertiary structures predicted with homology-based modelling were used to study the pore lining remains and to predict the solute transport activity of the AQPs. Expression profiling of the rose AQPs showed tissue-specific expression of many members. The data provided here will help in understanding the AQP-based solute transport system in roses and other related species.

KEYWORDS:

• Aquaporins
• Rosaceae
• rose
• solute specificity
• silicon

Acknowledgements

The authors are thankful to the “Department of Biotechnology (DBT), Government of India (GoI)”, for the “Ramalingaswami Fellowship Award” to R.D. and H.S. and grants BT/PR32853/AGIII/103/1159/2019 and BT/PR38279/GET/119/351/2020 to H.S.; The Science and Engineering Research Board (SERB), India, Department of Science and Technology (DST), Government of India (GoI), for research grant CRG/2019/006599 awarded to R.D., and H.S., and for research grant SRG/2021/000077 to SN. PR thanks the SERB, Govt. of India for the Project Associate-I fellowship. PK thanks the Director, ICAR-Directorate of Floricultural Research for funding support.

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/14620316.2023.2272153

Data availability statement

The authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.

Additional information

Funding

The work was supported by the Department of Biotechnology, Ministry of Science and Technology, India [BT/PR32853/AGIII/103/1159/2019]; Science and Engineering Research Board [SRG/2021/000077].