Phytochemical profiling and antioxidant activity of Linum trigynum

Abstract

In this study, the total phenolic and flavonoid contents (TPC and TFC), secondary metabolite composition (LC-HRMS/MS analyses) and antioxidant potential (DPPH, ABTS, GOR, CUPRAC, and phenanthroline assays) of Linum trigynum L. (LT) extracts were determined. Our results showed for the first time that the extracts (PE, CHCl₃, AcOEt, and n-BuOH) of LT exert antioxidant activity. The AcOEt and n-BuOH extracts were the most antioxidant compared to the standards, and had a higher amount of TPC (323.51 ± 0.62; 229.98 ± 6.80 µg GAE/mL) and TFC (183.75 ± 1.17 and 157.50 ± 1.77 µg QE/mL), resectively. The high antioxidant properties of these extracts may be due to their major compounds (phenolic compounds) detected by LC-HRMS/MS analyses including flavonoids (40 compounds) and phenolic acids and derivatives (18 and 19 compounds, respectively). AcOEt and n-BuOH extracts of LT can be used as an excellent source of antioxidant phytochemicals to prevent or treat various diseases.

Graphical Abstract

Keywords:

• Linum trigynum L
• antioxidant activity
• flavonoids
• phenolic acids
• analyses LC-HRMS/MS

Acknowledgments

The authors thank Laboratoire Radiopharmaceutiques Biocliniques (LRB), France, for financial support. The authors also thank Centre de Recherche en Biotechnologie (CRBt), Constantine, Algérie, helping us bring the present work to light.

Disclosure statement

The authors declare no conflicts of interest.

Correction Statement

This article has been corrected with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

The authors gratefully acknowledge DGRSDT-MESRS, Algeria and Laboratoire Radiopharmaceutiques Biocliniques (LRB), France for financial support.