Evaluation of genetic diversity among Saudi Arabian and Egyptian cultivars of alfalfa (Medicago sativa L.) using ISSR and SCoT markers

Abstract

Medicago sativa (Alfalfa) is valued for its high nutrient content as livestock feed for the production of dairy and meat products globally. The genetic improvement of alfalfa is limited because it is an allogamous autotetraploid. In this study, Inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) markers were used to assess the extent of genetic divergence between sixteen cultivars: twelve from Saudi Arabia and four from Egypt. The genetic diversity was assessed using eleven ISSR and eight SCoT primers. The ISSR primers produce 163 amplified bands with an average of 14.82 bands per primer and 60% polymorphism. The SCoT primers reveal 150 bands, with an average of 18.75 bands per primer and 77% polymorphism.  The dendrogram clustered the Saudi and Egyptian cultivars into two distinct populations. The information revealed here will help in the breeding program of alfalfa, which will produce more drought tolerant and high-yielding varieties.

KEYWORDS:

• Genetic diversity
• alfalfa
• ISSR
• SCoT Markers
• genetic improvement

Acknowledgements

The authors extend their thanks and appreciation to the University of Tabuk for providing the infrastructural support. Authors also acknowledge the personnel at Plant Gene Bank in the Ministry of Environment Water & Agriculture in Saudi Arabia and the Agriculture Research Center, Giza, Egypt for their work on sample collection and their kind cooperation.

Disclosure statement

No potential conflict of interest was reported by the author(s).