![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
ABSTRACT
This study tested whether an alloimmune response can occur in the marrow donor when infused or injected with leukocytes from their mixed chimeric transplant recipient. Two mixed chimeras were produced after conditioning with three Gray total body irradiation, donor marrow infusion, and post-grafting immunosuppression. The marrow donors were then repeatedly infused and injected with leukocytes from their respective chimeric recipient. A donor lymphocyte infusion (DLI) into their mixed chimeras had no effect, even after the experiments were repeated. The presence of blood dendritic cells (DCs) of recipient origin was confirmed in chimeric recipients, as well as the presence of microchimerism in the marrow donors. Donor sensitization did occur following placement of a recipient skin graft that was confirmed following DLI into recipients that changed the mixed chimeras into full donor chimeras. These observations suggest that mixed chimerism renders recipient peripheral blood DCs incapable of inducing a donor T cell response.
Disclosure of potential conflicts of interest
No potential conflicts of interest were disclosed.
Funding
The authors are grateful for research funding from the National Institutes of Health, Bethesda, MD, grants P01 CA078902, P30 CA015704, K12 CA076930, P30 DK056465, support from Gabrielle's Angel Foundation for Cancer Research (SLR), and by awards from the Joseph Steiner Krebsstiftung, Bern, Switzerland and Lupin Foundation, Metairie, LA (RS).
Author contributions
SLR designed experiments, performed the data analysis, and drafted the article. SSG and RS designed the experiments and drafted the article. DAH reviewed the article and assisted in data acquisition.