https://orcid.org/0000-0001-6571-1880
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ABSTRACT
Long non-coding RNA (LncRNA) AGAP2-AS1 has been demonstrated to involve in various malignancies. However, the expression and biological effect of AGAP2-AS1 on glioma remain enigmatic. We aimed to explore the effects of AGAP2-AS1 on glioma. Expressions and relationship of AGAP2-AS1 and microRNA-497-5p (miR-497-5p) in different grades of glioma tissues and cell lines as well as normal ones were assessed by quantitative real-time polymerase chain reaction (qRT-PCR), starBase, and dual-luciferase reporter assay. In addition, the effect of AGAP2-AS1 on the cell biological behaviors, epithelial-mesenchymal transition (EMT)-related markers, and miR-497-5p level was detected by cell functional experiments, western blot, and qRT-PCR. After transfection with miR-497-5p mimic (M), inhibitor (I), and AGAP2-AS1 knockdown, miR-497-5p level, cell biological behaviors, and EMT-related markers were detected again. AGAP2-AS1 expression was increased while miR-497-5p expression was decreased in glioma tissues and cell lines, and increase of AGAP2-AS1 expression or reduction of miR-497-5p expression was also correlated with clinicopathological grades of glioma. Furthermore, AGAP2-AS1 knockdown repressed cell biological behaviors and EMT-related markers expressions. Mechanically, AGAP2-AS1 targeted miR-497-5p and AGAP2-AS1 knockdown led to elevation of miR-497-5p expression. In addition, rescue experiments were conducted to validate the vital influence of miR-497-5p on the AGAP2-AS1-regulated proliferation and metastasis of glioma. AGAP2-AS1 may serve as an oncogene in the tumorigenesis of glioma by inhibiting miR-497-5p expression, showing its potential as a prognostic biomarker and a therapeutic target for glioma.
Highlight
1. AGAP2-AS1 was overexpressed in glioma tissues and cells.
2. AGAP2-AS1 silencing repressed cell biological behaviors and EMT process.
3. MiR-497-5p was lower expressed in glioma tissues and cells.
4. AGAP2-AS1 facilitated the progression of glioma by targeting miR-497-5p.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Availability of Data and Materials
The analyzed data sets generated during the study are available from the corresponding author on reasonable request.
Authors’ contributions
Substantial contributions to conception and design: Yi Sun
Data acquisition, data analysis and interpretation: Yulong Shen, Xing Li
Drafting the article or critically revising it for important intellectual content: Yi Sun
Final approval of the version to be published: Yi Sun, Yulong Shen, Xing Li
Agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of the work are appropriately investigated and resolved: Yi Sun, Yulong Shen, Xing Li