LncRNA FENDRR Suppresses Melanoma Growth via Influencing c-Myc mRNA Level

Abstract

Background

Long non-coding RNAs (lncRNAs) play an important role in the occurrence of melanoma. However, the specific molecular mechanisms that regulate its biological function are still poorly understood. Therefore, the main purpose of this study is to elucidate the internal mechanism of lncRNA-FENDRR as a biological marker for the occurrence of SKCM and its influence on its proliferation.

Results

FENDRR is low expressed in skin cutaneous melanoma (SKCM) tissues and appears to be at an even lower level as the tumor progresses. However, the high expression of FENDRR can affect the proliferation of SKCM cell line A375. The results of flow cytometry showed that after overexpression of FENDRR, the cell cycle was arrested in the G1/G0 phase. Bioinformatics analysis and RIP results showed that FENDRR could be combined with YTHDF1. Together, these complexes regulate c-Myc mRNA level and determine cell proliferation.

Conclusion

We found that overexpression of FENDRR can effectively inhibit SKCM, which provides a new theoretical basis for new therapeutic approaches and targeted RNA drugs.

Keywords:

• long non-coding RNAs
• FENDRR
• melanoma
• cell proliferation

Data Sharing Statement

Data were deposited at Mendeley: https://data.mendeley.com/datasets/kcgpw3wm6f/draft?a=370e05f4-85d3-4d1c-8b81-f05ab1159cec.

Ethical Approval

This study was approved by the Affiliated hospital of Weifang Medical College Ethics Committee, and the written informed consent of all participants was obtained before recruitment.

Author Contributions

All authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.

Disclosure

All the authors declared that there is no competing interest.

Additional information

Funding

This work was supported by Shandong Provincial Medical and Health Science and Technology Development Plan Project (No.202104101031).