Proteomic Analysis of Two Different Methods to Induce Skin Melanin Deposition Models in Guinea Pigs

Abstract

Objective

In this study, we analyzed the differential expression and key signaling pathways of proteins in the skin of guinea pigs with melanin deposition caused by two different modeling methods by utilizing proteomics techniques.

Methods

Guinea pig skin melanin deposition models were: (1) induced by ultraviolet (UV) irradiation alone (U group), (2) induced by UV combined with progesterone injection (P group), and guinea pigs treated without any treatment were used as blank group (B group). H&E staining and Masson staining were used to observe the extent of skin damage and melanin deposition in guinea pigs. The differentially expressed proteins (DEPs) in the skin tissues of melanin-deposited guinea pigs were screened by proteomic techniques, the functions of DEPs were analyzed, and a protein-protein interaction network (PPI) was constructed.

Results

There was a significant difference in grayscale between the U and P groups of guinea pig skin before and after modeling (P < 0.01). H&E and Masson staining showed that the U and P groups both exhibited incomplete keratinization of the stratum corneum, increased proliferation of epidermal cells with large nuclei and disordered arrangement, neovascularization of the dermis, and increased the number of melanin particles in the epidermis of the U and P groups of guinea pigs compared with the B group. Proteomics analysis showed that there were 171 DEPs between the U and P groups. These DEPs focused on biological processes such as fibrillar collagen trimer, extracellular matrix containing collagen proteins, metalloproteinase activity, and peroxidase activity.

Conclusion

The melanin pigmentation model induced solely by UV radiation negatively regulates biological processes such as extracellular matrix and collagen synthesis, while inducing significant skin photoaging. The combination of progesterone injections and UV radiation-induced melanin pigmentation model can cause abnormal protein expression in fatty acid and phospholipid metabolism, possibly being closer to the environment of melasma formation.

Keywords:

• melasma
• skin melanin
• melanin deposition
• ultraviolet
• progesterone

Data Sharing Statement

The data used to support the findings of this study are available from the corresponding author upon request.

Ethical Approval

The animal experiments were reviewed and approved by the Animal Ethics Committee of Ningxia Medical University (IACUC-NYLAC-2021-070).

Disclosure

Fei Song and Yan Wang contributed equally to this work as co-first authors. The authors declare that they have no competing interests.

Additional information

Funding

This work was supported by Ningxia Natural Science Foundation Project (2023AAC03158); National Natural Science Foundation of China Regional Science Foundation (81960893); Ningxia Medical University Special Talent Start-up Project (XT2022017).