Protective Effect of Panax notoginseng Extract Fermented by Four Different Saccharomyces cerevisiae Strains on H₂O₂ Induced Oxidative Stress in Skin Fibroblasts

Abstract

Purpose

To produce Panax notoginseng extract as a cosmetic ingredient through Saccharomyces cerevisiae fermentation.

Methods

We first compared the total sugar content, polysaccharide content, reducing sugar content, total phenolic content, total saponin content, DPPH free radical, ABTS free radical, hydroxyl free radical scavenging ability and ferric reducing antioxidant power (FRAP) of Panax notoginseng fermented extract (pnFE) and unfermented extract (pnWE). Their potential correlations were analyzed by Pearson’s correlation analysis. Then, the oxidative stress model of H₂O₂-induced MSFs was used to evaluate the effects of different pnFE on MSF viability, reactive oxygen species (ROS), malondialdehyde (MDA), and the activities of catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) to explore their protective effects on MSFs subjected to H₂O₂-induced cellular oxidative damage. Finally, their safety and stability were evaluated by using the red blood cell (RBC) test and hen’s egg test-chorioallantoic membrane (HET-CAM) assay, and changes in pH and content of soluble solids, respectively.

Results

Compared with pnWE, pnFE has more active substances and stronger antioxidant capacity. In addition, pnFE has a protective effect on H₂O₂-induced oxidative stress in MSFs with appropriate safety and stability.

Conclusion

PnFE has broad application prospects in the field of cosmetics.

Keywords:

• Panax notoginseng
• Saccharomyces cerevisiae
• fermentation
• oxidative stress
• fibroblast cells
• cosmetic ingredients

Disclosure

The authors report no conflicts of interest in this work.

Additional information

Funding

This research was funded by the State Administration for Market Regulation Science and Technology Plan Project (No. 2022MK192).