https://orcid.org/0000-0002-8655-0980
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Abstract
Background
Qiguiyin decoction (QGYD) was a traditional Chinese medicine (TCM) used to treat Pseudomonas aeruginosa infection in China. This study investigated the therapeutic effect and the potential mechanism of QGYD on carbapenem-resistant Pseudomonas aeruginosa (CRPA) infection.
Materials and Methods
Pulmonary infections were induced in mice by CRPA. The therapeutic effect of QGYD was evaluated by lung index and pulmonary pathology. The potential effects of QGYD on intestinal flora were detected by gut microbiome. The overall metabolism regulation of QGYD in blood was investigated by metabonomics. Next, the correlation between intestinal flora and metabolites was analyzed to illustrate the relationship between the regulatory effects of QGYD on metabolites and the beneficial effects of intestinal flora.
Results
QGYD has significant therapeutic effect on CRPA infection. QGYD profoundly inhibited the excessive accumulation of Deferribacteres and Mucispirillum at phylum and genus levels, respectively. Eleven potential metabolites that were abnormally expressed by CRPA infection and significantly reversed by QGYD were identified. Ten of the eleven metabolites significantly regulated by QGYD were related to Deferribacteres. Deferribacteres showed significant positive correlation with DL-lactic acid, phenylalanine and other metabolites and significant negative correlation with vitamin k1. At the genus level, Mucispirillum was closely related to metabolites significantly regulated by QGYD. Mucispirillum was positively correlated with metabolites such as Dl-lactate and negatively correlated with vitamin k1.
Conclusion
QGYD can improve CRPA infection and has the effect of regulating intestinal flora and metabolism. It was a promising drug against infection.
Abbreviations
CRPA, carbapenem-resistant Pseudomonas aeruginosa; CTAB, hexadecyl trimethyl ammonium bromide; NMDS, non-metric multidimensional scale; OTU, operational taxonomic units; PCoA, principal coordinate analysis; QGYD, Qiguiyin decoction; SPF, specific pathogen free; SD, standard deviation; TCM, traditional Chinese medicine.
Acknowledgments
This work was supported by grants from the National Traditional Chinese Medicine Multidisciplinary Innovation Team Project (ZYYCXTD-D-202201) and Natural Science Foundation of Capital Medical University (PYZ22166).
Disclosure
Professor Qingquan Liu reports a patent A new use of Chinese medicine licensed to CN102379931B. The authors report no other conflicts of interest in this work.