Abstract
Purpose
Pyroptosis, a new form of inflammatory programmed cell death, has recently gained attention. However, the impact of the expression levels of pyroptosis-related genes (PRGs) on the overall survival (OS) of osteosarcoma patients remains unclear. This study aims to investigate the impact of the expression levels of PRGs on the OS of pediatric and young adult patients with osteosarcoma.
Patients and Methods
Transcriptome matrix datasets of normal muscle or skeletal tissues from the Genotype-Tissue Expression (GTEx) project and osteosarcoma specimen the National Cancer Institute’s (NCI) Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database were used to identify pyroptosis-related genes (PRGs) associated with prognosis. The National Center for Biotechnology Information’s (NCBI) GSE21257 dataset was employed to validate the predictive value of the pyroptosis-related signature (PRS). Additionally, reverse transcription polymerase chain reaction (RT-qPCR) experiment was performed in normal and osteosarcoma cell lines.
Results
The study identified 18 differentially expressed PRGs (DEPRGs) between normal muscle or skeletal tissues and tumor samples. Multiple machine learning techniques were used to select PRGs, resulting in the identification of four hub PRGs. A PRS-score was calculated for each sample based on the expression of these four hub PRGs, and samples were categorized into low and high PRS-score level groups. It was confirmed that metastatic status and PRS-score level are independent prognostic predictors. A nomogram model for predicting OS of osteosarcoma patients was constructed. Single-cell RNA-sequencing data display the expression patterns of the hub PRGs. RT-qPCR data results were found to be consistent with the differential expression analysis performed on TARGET and GTEx samples.
Conclusion
The study developed a novel pyroptosis-related gene signature that can stratify pediatric and young adult osteosarcoma patients into different risk groups, thus predicting their response to immunotherapy and chemotherapy.
Data Sharing Statement
The datasets supporting the conclusions of this article are available in the following database: GDC portal (https://portal.gdc.cancer.gov/); the GTEx portal (https://gtexportal.org/home/); and the GEO database (https://www.ncbi.nlm.nih.gov/geo). R code scripts for the downstream analysis of the datasets: GTEx, TARGET-Osteosarcoma, GSE21257, and GSE162454, and the data generated during this study are available from the corresponding author on reasonable request.
Ethics Approval
The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Ethics Committee of Shanxi Provincial People’s Hospital [Protocol Code (2023) Sheng Yike Lun Shen Zi No. 457 of 28 January 2023].
Acknowledgments
We would like to thank the TARGET program, the GTEx project, and the GEO database for their free use.
Disclosure
The authors report no conflicts of interest in this work.