Identification and transcription profiling of NDUFS8 in Aedes taeniorhynchus (Diptera: Culicidae): developmental regulation and environmental response

Abstract

: The cDNA of a NADH dehydrogenase-ubiquinone Fe-S protein 8 subunit (NDUFS8) gene from Aedes (Ochlerotatus) taeniorhynchus Wiedemann has been cloned and sequenced. The 824 bp full-length mRNA sequence of AetNDUFS8 encodes an open reading region of 651 bp (that is, 217 amino acids). To identify if AetNDUFS8 is developmentally regulated, we employed a quantitative real-time polymerase chain reaction to examine AetNDUFS8 gene expression levels in all developmental stages of Ae. taeniorhynchus. In egg, larval, and pupal stages, AetNDUFS8 was expressed at relatively low levels. However, in the teneral adult stage, AetNDUFS8 was highly expressed. During the time course study in response to permethrin pesticide treatment, quantitative real-time polymerase chain reaction (PCR) also showed that mRNA transcription levels of AetNDUFS8 were regulated in female Ae. taeniorhynchus. A mitochondrially encoded NADH dehydrogenase subunit 5 AetNADH5 was highly expressed in different developmental stages of Ae. taeniorhynchus. This study suggests that AetNDUFS8 and AetNADH5 play an essential role in the development of Ae. taeniorhynchus and will provide information useful for developing dsRNA pesticide for mosquito control.

Keywords::

• Aedes taeniorhynchus
• AetNDUFS8
• mRNA expression
• development
• permethrin

Acknowledgments

We thank Drs SM Valles (USDA-ARS) and L Zhang (Delta Research and Extension Center, Mississippi State University) for critical reviews of the manuscript. We also thank Neil Sanscrainte and Kelly Anderson (USDA-ARS) for their helpful support. The current study was supported by a grant from the Deployed War-Fighter Protection Research Program funded by the US Department of Defense through the Armed Forces Pest Management Board.

Disclosure

The authors report no conflicts of interest in this work.

Supplementary materials

Table S1 Expression of AetNDUFS8 in different developmental stages of Aedes taeniorhynchus

Table S2 Paired t-test data for comparison of relative AetNDUFS8 gene transcription between female (F) and male (M), as well as different ages between the same sex (either female or male) in Aedes taeniorhynchus

Table S3 Paired t-test data for comparison of relative AetNDUFS8 gene transcription between eggs, larvae, pupae, and adults in Aedes taeniorhynchus

Table S4 Expression of AetNDUFS8 under permethrin stress conditions in Aedes taeniorhynchus

Table S5 Paired t-test data for comparison of relative AetNDUFS8 gene transcription between acetone, low dose (LD), and high dose (HD) permethrin treatments in Aedes taeniorhynchus

Table S6 Expression of AetNADH5 in different developmental stages of Aedes taeniorhynchus

Table S7 Paired t-test data for comparison of relative AetNADH5 gene transcription between female (F) and male (M), as well as different ages between the same sex (either female or male) in Aedes taeniorhynchus

Table S8 Paired t-test data for comparison of relative AetNADH5 gene transcription between eggs, larvae, pupae, and adults in Aedes taeniorhynchus