Effects of soyhulls with different particle size on the growth performance, blood indices and gut microbiota of yellow feather broilers

ABSTRACT

1. The objective of this study was to determine the effects of soyhulls with different particle sizes on the growth performance, blood indices and gut microbiota of yellow feather broilers.

2. Total of 240 healthy, one-day-old, yellow feather broilers were randomly divided into four groups, with six pen replicates within each group and ten birds per pen. The control group birds were fed the basal diet (Control). For the treatment groups, 5% soyhulls with different particle sizes were included in the basal diet. The particle size geometric mean diameters (d_gw) of the soyhulls in the three treatment groups were 299.69 μm (LowPS), 489.85 μm (MediumPS) and 734.83 μm (HighPS) with geometric standard deviation (S_gw) 1.75 μm, 1.62 μm and 1.67 μm, respectively.

3. Results showed that the growth performance variables and organ indices were not different among the four groups. The MediumPS group had increased TG, T-CHO, ALT, HDL-C, and GSH-PX levels and decreased T-AOC levels, whereas LowPS and HighPS groups had increased HDL-C and GSH-PX levels (p < 0.05). Microbial diversity analysis showed that the intestinal microbiota of yellow feather broilers mainly included Firmicutes and Bacteroidetes. Inclusion of 5% soyhulls with different particle size had no effect on alpha diversity indices of caecal microbiota. The HighPS group had significantly higher relative abundance of Firmicutes spp. and lower Bacteroidetes spp. compared with the LowPS and MediumPS group but this was not different from the Control group. The relative abundance of Cyanobacteria spp. was significantly higher in the HighPS group than the other three groups. LEfSe analysis showed that there were more enriched biomarker taxa in the groups with soyhulls than the control group.

4. Overall, the inclusion of soyhulls with different particle sizes had limited effects on growth performance, blood indices and caecal microbiota composition of yellow feather broilers.

KEYWORDS:

• Soyhulls
• particle size
• growth
• blood
• microbiota

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The data that support the findings of this study (Reference No. PRJNA973843) can be found at https://www.ncbi.nlm.nih.gov/bioproject/?term=1010995.

Additional information

Funding

The financial support from Guangdong Basic and Applied Basic Research Foundation [2019A1515110780], Discipline Construction Program of Foshan University [CGZ0400162], the research start-up fund for Postdoctoral Fellows from Foshan City (BKS209059), the Scientific research start-up fund for high-level talents of Foshan University (Gg07145), the National Natural Science Foundation of China [Grant No. 31902228] were confirmed.