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Australian Journal of Forensic Sciences Volume 56, 2024 - Issue sup1: Extended Abstracts of the 23rd IAFS, incorporating the 26th ANZFSS, Conference
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Abstract

Optimization of flow cytometry methods to differentiate epithelial cell origin

Ishana Somersa College of Science and Engineering, Flinders University, Bedford Park, Australia
,
Amy Brittona College of Science and Engineering, Flinders University, Bedford Park, Australia
,
Giles Bestb Flinders Health and Medical Research Institute, Flinders University Flow Cytometry Facility, Bedford Park, Australia
,
Duncan Taylora College of Science and Engineering, Flinders University, Bedford Park, Australia;c Forensic Science SA, Adelaide, AustraliaORCID Iconhttps://orcid.org/0000-0003-0633-7424
ORCID Icon,
Roland A.H. van Oorschotd Office of the Chief Forensic Scientist, Victoria Police Forensic Services Department, Macleod, Australia;e School of Agriculture, Biomedicine and Environment, La Trobe University, Bundoora, Australia
&
Mariya Goraya College of Science and Engineering, Flinders University, Bedford Park, AustraliaCorrespondence[email protected]
Pages 135-138 | Received 30 Jan 2024, Accepted 04 Feb 2024, Published online: 28 Apr 2024
 
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1

ABSTRACT

The ability to link a DNA profile to a source of biological material has significant implications in forensic investigations. Epithelial cells are the main constituent of many of the samples collected as part of forensic casework. However, it is currently not possible to distinguish between or isolate epithelial cells from different anatomical regions. Flow cytometry represents a potential method that could be used in this context. This study was conducted to optimize procedures for the collection and processing of epidermal, buccal, vaginal and penile epithelial cells prior to analysis by flow cytometry.

Disclosure statement

No potential conflict of interest was reported by the author(s).

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