Cytotoxicity of Sarcosphaera crassa and Tricholoma terreum extracts on colon cancer cell line (HT-29) in conjunction with their antioxidant properties

ABSTRACT

The purpose of this study was to investigate and compare the antioxidant and cytotoxic activities of Sarcosphaera crassa and Tricholoma terreum extracts. Phenolic compounds analysis of both mushroom extracts has been carried out by HPLC. Antioxidant activities of the extracts were tested by using in vitro assays. The cytotoxicity of extracts on HT-29 cell line were determined by the WST assay. The total phenolic and total flavonoid contents of T. terreum extracts were higher than those S. crassa. Also, we detected that T. terreum extracts had higher total antioxidant activity and scavenging ability on DPPH radicals than S. crassa extracts. However, the T. terreum methanol extracts showed 27% cytotoxic effect in HT-29 cell line whereas the ethanolic extracts of S. crassa showed cytotoxicity 45%. These results indicate that both mushroom extracts have considerable cytotoxic and antioxidant properties and may be utilized as a promising source of therapeutics.

KEYWORDS:

• Antioxidant
• cytotoxicity
• Sarcosphaera crassa and Tricholoma terreum

Acknowledgments

The authors wish to thank to the Scientific Research Projects Unit of Erciyes University for financial supporting (Project number: TSA-11-3580). The authors would also like to thank to Prof. Dr. Belma Aslim, Assoc. Prof. Hakan Alli and Assist. Prof. Berrak Altınsoy for their helps.

Disclosure statement

No conflict of interest associated with this work.

Additional information

Funding

This work was supported by the The Scientific Research Projects Unit of Erciyes University [Project number: TSA-11-3580].