Chemical composition, antimicrobial and antiproliferative activity of the essential oil from Ambrosia artemisiifolia L

ABSTRACT

The aim of our study was to characterize the composition and certain bioactivities of the essential oil of Ambrosia artemisiifolia. Eighty organic compounds were identified in the essential oils obtained by hydrodistillation and microwave-assisted isolation. Germacrene D was the main component in both oils. The essential oil obtained by hydrodistillation exerted antibacterial activity against two S. aureus strains, with MIC values of 0.015% for the methicillin-susceptible strain and 0.25% for the methicillin-resistant strain. In the biofilm formation assay, a dose-dependent inhibition was observed in case of the K. pneumoniae ATCC 600,703. In the ethidium bromide accumulation assay, a dose-dependent inhibition activity was observed against the bacterial efflux pump of the Gram-negative bacteria E. coli ATCC 25,922. The essential oil displayed strong in vitro cytotoxicity against the multidrug-resistant (MDR) Colo 320 cancer cell line with an IC₅₀ value of 0.0103%. The essential oil showed strong inhibition on the proliferation of the doxorubicin-sensitive Colo 205 cells (IC₅₀ values of 0.054%) and in the case of MDR Colo 320 colonic adenocarcinoma cells (IC₅₀ values of 0.008%). This is the first report of the cytotoxic and antiproliferative activities of the essential oil from these species.

KEYWORDS:

• Asteraceae
• Ambrosia artemisiifolia
• essential oil
• hydrodistillation
• microwave-assisted isolation
• antibacterial activity

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/10412905.2024.2303449