ABSTRACT
Worldwide thin layer chromatography (TLC) is still in very much use in various different application fields (e.g. natural products chemistry, monitoring the organic/enantioselective synthesis, and routine analysis in analytical laboratories). Herein is presented an account of the efficient resolution/enantioseparation of racemic and non-racemic mixtures of several different types of active pharmaceutical ingredients, and biologically important compounds by TLC using a direct approach along with quantitative determination and isolation of native forms via non-covalent diastereomer formation. Different approaches and mechanisms of direct enantioseparation using different chiral reagents are discussed. It provides a route for a wide range of chiral stationary phases (CSPs) which can be in-home thought out, produced, assessed, and customized. The achievements and new challenges in the field are presented. Besides, it presents a very useful coverage for the non-specialists to the subject who want to understand where this study fits into the existing literature in this general area.
Acknowledgments
The authors are thankful to the Alexander von Humboldt-Stiftung, Bonn, Germany for a research grant.
Disclosure Statement
No potential conflict of interest was reported by the authors.
Correction Statement
This article has been corrected with minor changes. These changes do not impact the academic content of the article.