ABSTRACT
Environmental damage is caused by the waste produced by slaughterhouses. The leftovers from slaughterhouses make a solid base for the production of clean energy. In the present study, biohydrogen was produced using the cattle rumen fluid (RF) collected from the slaughterhouse. The RF serves as the only substrate, and 21.13 ± 0.176 µmol H2 ml−1rumen fluid (RF) was found to be produced during biohydrogen synthesis. After 72 h, the rumen fluid was collected and a pure culture of bacteria that produce biohydrogen was isolated. After characterization, the organism was identified as Staphylococcus sciuri MK898925.1. The Sterilised RF was used as the sole substrate for biohydrogen production by Staphylococcus sciuri MK898925.1, and biohydrogen production was found to be 142.4 ± 2.37 µmol H2 ml−1 RF. Staphylococcus sciuri MK898925.1 was screened for the presence of the gene hydrogenases and it was discovered that the gene was present in the bacteria. The bacteria Staphylococcus sciuri MK898925.1 isolated from the rumen fluid of cattle can be used to produce biohydrogen. A large amount of slaughterhouse waste rumen fluid can be recycled and converted into energy at a very low cost.
Acknowledgements
The authors are thankful to the Department of Animal Biotechnology, Faculty of Basic Sciences, Madras Veterinary College, Chennai, India, for all the research facilities provided and to TAMIL NADU INNOVATIVE INITIATIVES (TANII), Government of Tamil Nadu, India, for funding the project.
Disclosure statement
No potential conflict of interest was reported by the authors.
Additional information
Notes on contributors
Merli Maman
Merli Maman has completed a Ph.D. in Biotechnology from Tamil Nadu Veterinary and Animal Sciences University, India. She has worked on biohydrogen production from rumen microbes and published articles in reputed journals.
Meignanalakshmi Sundaram
Dr. Meignanalakshmi Sundaram is a professor in the Department of Animal Biotechnology at Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Chennai, Tamil Nadu, India. After completing her M.V.Sc. and Ph.D. in Animal biotechnology, she has more than 23 years of teaching and research experience in Animal Biotechnology, and her specialization areas are rumen microbiology, genome analysis, bioelectricity, bioplastics, and Environmental biotechnology. She has mentored M.V.Sc., M.Phil., M.Tech, M.Sc and Ph.D. students as Chairman of the Advisory Committee. She has many National and International publications.
Vinitha Vivekanandan
Vinitha Vivekanandan has completed a Ph.D. in Biotechnology from Tamil Nadu Veterinary and Animal Sciences University, India. She has worked on biodiesel production from microalgae and has published articles in highly regarded journals.