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Genetic Resources Evaluation

Physiological and candidate gene analysis of RG5, a locus associated with a higher ratio of filled grains in rice (Oryza sativa L.)

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Pages 150-154 | Received 31 Aug 2023, Accepted 03 Mar 2024, Published online: 29 Mar 2024

Figures & data

Figure 1. (a) Two hypothetical models for high starch content by RG5. Two hypotheses were consideredregarding the function of RG5 (1) the upper part might be altered to be sink, and (b) the sink capacity of thelower part might be improved. (a) and the manner of accumulated starch in Koshihikari (white box) andSLRG5 (black box) (b). Third leaf sheath was separated into 5 parts and used. The contents of starch weresignificant different in all parts between them (p <0.001).

Figure 1. (a) Two hypothetical models for high starch content by RG5. Two hypotheses were consideredregarding the function of RG5 (1) the upper part might be altered to be sink, and (b) the sink capacity of thelower part might be improved. (a) and the manner of accumulated starch in Koshihikari (white box) andSLRG5 (black box) (b). Third leaf sheath was separated into 5 parts and used. The contents of starch weresignificant different in all parts between them (p <0.001).

Figure 2. (a) Relative expression of key genes for starch synthase in 5th (lower) part of third sheath and (b)third leaf area of Koshihikari (white box) and SLRG5 (black box). BE1:branching enzyme 1; SuS: sucrose synthase. *means P<0.05

Figure 2. (a) Relative expression of key genes for starch synthase in 5th (lower) part of third sheath and (b)third leaf area of Koshihikari (white box) and SLRG5 (black box). BE1:branching enzyme 1; SuS: sucrose synthase. *means P<0.05

Figure 3. Fine mapping of RG5 in rice. Black and white line mean chromosome of Kasalath andKoshihikari, respectively. In phenotype data, blue box indicated grain number per a panicle.Gray box did the ratio of filled grain (%) without 3-E-6.All markers were listed in supplemental table 3.

Figure 3. Fine mapping of RG5 in rice. Black and white line mean chromosome of Kasalath andKoshihikari, respectively. In phenotype data, blue box indicated grain number per a panicle.Gray box did the ratio of filled grain (%) without 3-E-6.All markers were listed in supplemental table 3.

Table 1. Population of Koshihikari and Kasalath type allele in rice.

Supplemental material

Supplemental Material

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