Hepatitis B virus infection seromarkers among college freshmen and their immune responses to different vaccination policies of hepatitis B vaccine

Figures & data

Table 1. Distribution of HBsAg and anti-HBs positive rate among college freshmen

Group	HBsAg				HBsAb
	Total No. of subjects	No. of positive (N)	Positive rate (%,95%CI)	χ^2, P^a	No. of subjects	No. of strong Positive(N)	Strong Positive rate (%,95%CI)	No. of weak Positive(N)	Week positive rate (%,95%CI)	χ^2, P^a
Age(years)				χ^2 = 7.574, P = .023						χ^2 = 26.531, P < .001
≤18	798	5	0.6(0.1–1.2)		793	173	21.8(18.9–24.7)	123	15.5(13.0–18.0)
19	7181	33	0.5(0.3–0.6)		7148	1136	15.9(15.0–16.7)	1001	14.0(13.2–14.8)
≥20	2666	25	0.9(0.6–1.4)		2641	397	15.0(13.7–16.4)	402	15.2(13.8–16.6)
Gender				χ^2 = 2.456, P = .117						χ^2 = 25.439, P < .001
male	6059	42	0.7(0.5–0.9)		6017	1064	17.7(16.7–18.6)	861	14.3(13.4–15.2)
female	4586	21	0.5(0.3–0.7)		4565	642	14.1(13.1–15.1)	665	14.6(13.5–15.6)
Region				χ^2 = 8.224, P = .004						χ^2 = 39.957, P < .001
local	5796	23	0.4(0.2–0.6)		5773	819	14.2(13.3–15.1)	813	14.1(13.2–15.0)
non-local	4849	40	0.8(0.6–1.1)		4809	887	18.4(17.3–19.5)	713	14.8(13.8–15.8)
Total	10645	63	0.6(0.4–0.7)		10582	1706	16.1(15.4–16.8)	1526	14.4(13.8–15.1)

^aP valves were based on Chi-Square analyses for categorical variables.

Table 2. Rates of anti-HBs seroconversion and GMT levels of anti-HBs after HepB vaccination in college freshmen among various groups via univariate analysis

Variables	Total No. of subjects	anti-HBs sero-conversion (N,%)	χ^2/t, P	anti-HBs GMT (mIU/mL)	t/F, P
Gender			χ^2 = 5.119, P = .024		t = 2.762, P = .006
Male	567	556(98.1)		845.33 ± 278.68
Female	719	689(95.8)		799.53 ± 315.04
Age			χ^2 = 12.009, P = .002		F = 0.809, P = .445
≤18	734	714(97.3)		823.47 ± 294.91
19	435	424(97.5)		822.44 ± 300.93
≥20	117	107(91.5)		786.07 ± 330.67
National			χ^2 = 0.775, P = .379		t = 0.556, P = .578
Han nationality	1228	1190(96.9)		820.73 ± 298.62
Other nationalities	58	55(94.8)		798.28 ± 336.04
Registration			χ^2 = 2.421, P = .298		F = 1.509, P = .222
Hangzhou City	158	156(98.7)		845.76 ± 274.37
Other cities in Zhejiang province	769	741(96.4)		823.95 ± 299.86
Other provinces	359	348(96.9)		799.21 ± 311.49
BMI(kg/m^2)			χ^2 = 0.015, P = .993		F = 0.736, P = .479
<24	1130	1094(96.8)		816.22 ± 301.13
24–28	128	124(96.9)		839.97 ± 297.19
>28	28	27(96.4)		868.48 ± 282.75
History of allergy			χ^2 = 6.550, P = .010		t = −1.904, P = .058
Yes	218	205(94.0)		781.63 ± 330.17
No	1068	1040(97.4)		827.50 ± 293.39
History of HepB vaccination			χ^2 = 8.547, P = .014		F = 1.546, P = .213
Vaccinated	439	417(95.0)		799.93 ± 321.23
Unvaccinated	126	121(96.0)		819.68 ± 296.36
Unknown	721	707(98.1)		831.85 ± 287.21
Hep B vaccination doses			χ^2 = 236.944, P < .001		t = −13.333, P < .001
1 dose	195	154(79.0)		502.44 ± 377.82
3 doses	1091	1091(100.0)		876.43 ± 244.30
Total	1286	1245(96.8)		819.72 ± 300.30

A value of P < .05 (2-sided) was considered statistically significant.The significance of bold values in Table 2 considered statistically significant

Figure 1. Current diseases of college freshmen (a), past diseases of college freshmen (b), and family history of diseases of college freshmen (c).

Table 3. Association of Seroepidemiology of anti-HBs and related variables via multivariate analysis

The independent variables	anti-HBs GMT (mIU/mL) = y^a
	OR (95% CI)	P
Hep B vaccination doses
1 dose	1
3 doses	1.58 (1.51–1.65)	<.001
Gender
Male	1
Female	0.94 (0.89–0.99)	.026
Registration
Hangzhou City	1
Other cities in Zhejiang province	0.94 (0.90–0.99)	.024
Other provinces	0.93 (0.88–0.98)	.011

^aLinear regression was used to analyze the associations between the GMT levels of anti-HBs and the related factors adjusted age.