Figures & data
Figure 1. Representative images of the protein expression of iNOS or nitrotyrosine in normal urinary bladder in rat. (A) The bladder specimen was stained without primary antibody against iNOS (negative control; Neg), resulting in no immunoreactivity. (B) Application of the Santa Cruz anti-iNOS antibody to the rat bladder specimen demonstrated immunoreactivity in the urothelium (U) and to a lesser extent also in the lamina propria (Lp) and the detrusor muscle (D). (C) Application of the Santa Cruz anti-iNOS antibody to the bladder specimen, after incubation of the antibody with its immunogenic peptide (‘blocking peptide’) markedly attenuated the immunoreactivity. (D) Application of the anti-nitrotyrosine antibody to the bladder specimen demonstrated immunoreactivity in the urothelium (U) and leaving the lamina propria (Lp) essentially unstained. Scale bars: 50 µm.
Figure 2. Application of the anti-iNOS antibody from Santa Cruz elicited a perinuclear immunoreactivity in the detrusor (arrowhead). Inlet: immunoreactivity noted in the lamina propria of the urinary bladder. Scale bar: 20 µm.
