Preparation and exchange transfusion effect of a double polymerization human umbilical cord haemoglobin of red blood cell substitute

Figures & data

Figure 1. Schematic diagram of experimental grouping. C: control group; D: GDA-DBBF-HCHb group; G: GDA-HCHb group.

Table 1. Comparison of the main physical and chemical indicators of the two groups of products.

Detect items	Groups
	DBBF-GDA-HCHb group	GDA-HCHb group
Dimer (%)	2.1	4.0
Tetramer (%)	26.3	5.6
Super weight molecule (>600 kD) (%)	1.1	1.9
Effective polymerization ratio (%)	97.9	96.0
Average molecular weight (kD)	237.7	342.5
pH	7.84	7.85
Concentration (g/dL)	6.0	6.0
MetHb (%)	3.5	3.1
P50 (mmHg)	18.2	9.5

Table 2. Comparison of the main physical and chemical indicators of the two groups of products at 0 point and after 2 months.

Detect items	0 Point		After 2 months
	DBBF-GDA- HCHb	GDA-HCHb	DBBF-GDA- HCHb	GDA-HCHb
Dimer (%)	1.9 ± 0.2	3.7 ± 0.2	1.9 ± 0.2	3.9 ± 0.3
Tetramer (%)	24.7 ± 1.9	5.4 ± 0.4	23.7 ± 2.2	5.3 ± 0.4
Concentration (g/dL)	5.9 ± 0.2	6.1 ± 0.2	5.9 ± 0.1	6.1 ± 0.2
MetHb (%)	3.5 ± 0.3	3.0 ± 0.1	3.4 ± 0.2	3.0 ± 0.3
P50 (mmHg)	18.1 ± 0.2	9.9 ± 0.6	18.0 ± 0.2	10.0 ± 0.6

Figure 2. Molecular weight distribution of DBBF cross-linked HCHb at different pH (n = 3).

Figure 3. Molecular weight distribution of DBBF cross-linked HCHb under different IHP concentrations (C_IHP) (n = 3).

Figure 4. The molecular weight distribution of cross-linked HCHb under different mole ratios of DBBF and HCHb (Mole Ratio_DBBF:HCHb).

Figure 5. The P50 and Hill co (A) and MetHb (B) of DBBF-HCHb under different Mole ratios_{DBBF: HCHb} (n = 3).

Figure 6. Molecular weight distribution of GDA-DBBF-HCHb under different mole ratios of GDA and DBBF-HCHb (n = 3).

Figure 7. The P50 and Hill co (A) and MetHb (B) of DBBF-HCHb under different Mole ratios_{GDA:DBBF-HCHb} (n = 3).

Figure 8. Comparison of percent survival within 12 h of three groups of rats after 135% exchange transfusion (n = 6).

Figure 9. (A) TCO₂, (B) PCO₂, (C) HCO₃⁻, (D) HCO_3STD change at baseline, end of the ET (End), and after 2 h of ET (After 2 h) in three groups of rats (n = 6).

Figure 10. (A) HR and (B) MAP change at baseline, end, and after 2 h in three groups of rats (n = 6).

Figure 11. PO₂ change at baseline, end of the exchange, and after 2 h of exchange in three groups of rats (n = 6).

Figure 12. (A) BEecf, (B) BE and (C) Lac change at baseline, end, and after 2 h in three groups of rats (n = 6).

Data availability statement

The authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.