https://orcid.org/0000-0003-1415-4169
Figures & data
Table 1. Genotypic and phenotypic characteristics of OXA-48-like-producing P. mirabilis isolates from this study.
Figure 1. Maximum-likelihood cgMLST-based phylogeny of OXA-48-like-producing P. mirabilis from this study and external sequences from GenBank. The distance of the tree branches represents allelic differences in the cgMLST scheme (in total 2,334 loci). Nodes with close epidemiological relationship were combined in one node (cut-off: 12 allelic differences). Isolates with the same ST type are indicated by dotted circles. Within the same ST type, genomic distances are also expressed as SNPs from whole genome phylogeny (red numbers).
Figure 2. Variants of genetic environments of blaOXA-48 in P. mirabilis compared to the plasmid pOXA-48-PM. Grey arrows indicate mobile genetic elements or associated genes. Unlabelled arrows indicate genes coding for hypothetical proteins. IRL/IRR = left and right inverted repeats. TSD = target site duplications.
Figure 3. Graphical representation of the novel genomic islands PmGRI1-P1/P6 (a) and PmGRI1-P3 and the plasmid pOXA-P10 (b). Genetic structures are colour encoded according to the legend. Grey areas between the structures indicate regions of high sequence homology. Numbers below the name label describe the isolation date of the according isolate.
Figure 4. Plasmid variants of pOXA-48 found in two P. mirabilis isolates. Grey arrows = mobile genetic elements, red arrows = antibiotic resistance genes, green arrows = other genes or open reading frames. Unlabelled arrows indicate genes coding for hypothetical proteins.
Figure 5. The novel MOBF plasmid pOXA-P12, harbouring blaOXA-181 within the novel transposon Tn7713. Red arrow = blaOXA-48, grey arrows = other parts of Tn7713, green arrows = genes associated with plasmid replication, blue arrows = genes associated with plasmid transfer, yellow arrows = genes coding for hypothetical proteins.
Figure 6. Tn6360-like variants in blaOXA-181-harbouring P. mirabilis. Grey arrows = mobile genetic elements or associated genes, red arrows = antibiotic resistance genes, blue arrows = other genes or gene fragments, white arrows = genes outside of the transposon structure. IRL/IRR = left and right inverted repeats, TSD = target site duplications.
Figure 7. Minimal inhibitory concentrations (MICs) of piperacillin-tazobactam, ertapenem and meropenem for OXA-48-producing transconjugants. Transconjugants comprise P. mirabilis ARP (red dots, n = 6) and E. coli J53 (blue squares, n = 6), sharing isogenic pOXA-48-like plasmid variants. Black bars indicate the median. Two asterisks indicate a statistically significant difference of p ≤ 0.01.
