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Food Science & Technology

Profiling the potential producers of higher alcohol in different Daqu (a starter of Baijiu) by amplifying the key enzyme gene

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Article: 2306014 | Received 13 Sep 2023, Accepted 10 Jan 2024, Published online: 27 Jan 2024

Figures & data

Figure 1. The metabolic pathway of higher alcohol in the microorganism.

(A) Ehrlich pathway and Biosynthesis pathway, (B) Amino acid transformation pathway, and (C) Wood-Ljungdahl pathway. ADH is the abbreviation of alcohol dehydrogenase.

Figure 1. The metabolic pathway of higher alcohol in the microorganism.(A) Ehrlich pathway and Biosynthesis pathway, (B) Amino acid transformation pathway, and (C) Wood-Ljungdahl pathway. ADH is the abbreviation of alcohol dehydrogenase.

Figure 2. Design and verification of degenerate primers.

(A) WebLogo of the amino acid sequences of 51 L.ADH, (B) Degenerate primers, and (C) the electrophoretogram of the amplification by degenerate primer.

Figure 2. Design and verification of degenerate primers.(A) WebLogo of the amino acid sequences of 51 L.ADH, (B) Degenerate primers, and (C) the electrophoretogram of the amplification by degenerate primer.

Table 1. Sequencing library and fragment length.

Table 2. The diversity of the L.adh gene fragments of Daqu.

Figure 3. Annotation of the potential producers of higher alcohol of different Daqu in genus level.

(A) Library 1, (B) Library 2, and (C) Library 3.

Figure 3. Annotation of the potential producers of higher alcohol of different Daqu in genus level.(A) Library 1, (B) Library 2, and (C) Library 3.

Figure 4. Annotation of the potential producers of higher alcohol of different Daqu in species level.

Figure 4. Annotation of the potential producers of higher alcohol of different Daqu in species level.

Figure 5. Phylogenetic tree of L.adh gene fragments of Daqu.

(A) HT, (B) SC, and (C) SD.

Figure 5. Phylogenetic tree of L.adh gene fragments of Daqu.(A) HT, (B) SC, and (C) SD.

Table 3. Sequencing results of 97% similarity of the L.adh gene fragments of Daqu.

Supplemental material