Estimation of ODAP contents and heritability of quantitative traits in grass pea (Lathyrus sativus L.) accessions from North-Western Ethiopia

Figures & data

Table 1. The grass pea accessions used in the study with zonal, district, latitudinal, longitudinal, and altitudinal records.

No	Accessions	Zone	District	Latitude	Longitude	Altitude
1	238935	South_Gondar	Fogera	11°-55ʹ-24ʺ-N	37°-54ʹ-21ʺ-E	2130.00
2	236702	South_Gondar	Dera	–	–	1800.00
3	212741	South_Gondar	Kemekem	12°-06ʹ-00ʺ-N	37°-42ʹ-00ʺ-E	2000.00
4	226014	South_Gondar	Este	11°-27ʹ-00ʺ-N	37°-59ʺ-00ʹ-E	2645.00
5	238931	South_Gondar	Fogera	11°-57ʹ-45ʺ-N	37°-43ʹ-35ʺ-E	1920.00
6	226011	South_Gondar	Este	11°-03ʹ-00ʺ-N	38°-09ʹ-00ʺ-E	2520.00
7	226017	North_Gondar	Dembia	12°-30ʹ-00ʺ-N	37°-24ʹ-00ʺ-E	1905.00
8	226018	North_Gondar	Gondar zuria	12°-31ʹ-00ʺ-N	37°-20ʹ-00ʺ-E	1990.00
9	236708	North_Gondar	Dabat	–	–	2730.00
10	238928	North_Gondar	Gondar zuria	12°-24ʹ-01ʺ-N	37°-33ʹ-09ʺ-E	1990.00
11	242216	North_Gondar	Gondar zuria	12°-30ʹ-00ʺ-N	37°-32ʹ-00ʺ-E	1975.00
12	46107	North_Gondar	Gondar zuria	12°-37ʹ-00ʺ-N	37°-10ʹ-00ʺ-E	1950.00
13	236697	West_Gojjam	Adet	–	–	2260.00
14	236712	West_Gojjam	Jabi Tehnan	–	–	1820.00
15	238947	West_Gojjam	Dega Damot	10°-38ʹ-55ʺ-N	37°-23ʹ-58ʺ-E	1900.00
16	238920	West_Gojjam	Merawi	11°-25ʹ-09ʺ-N	37°-09ʹ-54ʺ-E	2050.00
17	238942	West_Gojjam	Achefer	11°-44ʹ-33ʺ-N	36°-59ʹ-06ʺ-E	2050.00
18	238945	West_Gojjam	Achefer	11°-48ʹ-02ʺ-N	36°-59ʹ-59ʺ-E	2030.00
19	24812	East_Gojjam	Hulet Ej Enese	11°-05ʹ-45ʺ-N	37°-52ʹ-28ʺ-E	2452.00
20	30357	East_Gojjam	Enarj Enawaga	10°-40ʹ-57ʺ-N	38°-11ʹ-13ʺ-E	2517.00
21	238908	East_Gojjam	Dejen	09°-57ʹ-28ʺ-N	38°-18ʹ-26ʺ-E	2450.00
22	26626	East_Gojjam	Debay Telatgen	10°-25ʹ-26ʺ-N	38°-07ʹ-33ʺ-E	2573.00
23	26627	East_Gojjam	Baso Liben	10°-04ʹ-44ʺ-N	37°-44ʹ-54ʺ-E	2350.00
24	238910	East_Gojjam	Enemay	10°-21ʹ-29ʺ-N	38°-09ʹ-36ʺ-E	2440.00
25	26633	East_Gojjam	Awabel	10°-14ʹ-14ʺ-N	38°-03ʹ-24ʺ-E	2439.00

Table 2. Analysis of variance (mean square) of 13 quantitative and one quality trait in 25 grass pea accessions grown at EBSU, CAS campus (2019).

	Mean Square
		Treatment			Error
Source of Variation	Rep	Unadjusted	Adjusted	Block within Replication	Intra block	RCBD	R^2 %	Efficiency relative to RCBD
Df	1	24	24	8	16	24
DE	0.98	2.00	1.59*	0.31	0.57	0.48	84.97	84.58
DF	1.28	260.95	223.44***	4.98	5.18	5.11	98.70	98.71
NPB	0.23	1.37	1.22**	0.15	0.35	0.29	85.79	81.16
NSB	4.15	118.65	97.01***	4.66	1.00	2.22	99.45	175.42
NBPP	6.34	128.13	103.46***	4.38	0.93	2.08	99.52	177.10
PH	241.12	150.04	123.85***	11.09	13.02	12.37	94.97	95.05
PBL	236.14	146.63	121.65***	10.83	12.95	12.25	94.88	94.54
LBB	0.03	0.57	0.46***	0.03	0.04	0.04	95.13	90.18
NNPB	0.08	17.31	16.58***	2.60	2.10	2.27	92.86	101.44
PBINL	0.04	0.10	0.09***	0.02	0.01	0.02	92.28	107.67
LL	0.0005	0.11	0.08**	0.01	0.02	0.02	89.90	88.22
PtL	0.0001	0.017	0.02 ns	0.008	0.009	0.009	76.71	97.53
LW	0.0002	0.005	0.005***	0.002	0.0004	0.0008	95.54	166.89
ODAP	0.002	0.052	0.042***	0.0003	0.0003	0.0003	99.60	96.73

Note: *** indicates very highly significant at <0.001 probability level, ** highly significant at probability level <0.01, * significant at probability level < 0.05, ns: non-significant difference, Df: degree of freedom, DE: Days to emergence, DF: Days to flowering, NPB (No.): the number of primary branches, NSB(No): number of secondary branches, NBPP(No): number of branches per plant, PH(cm): Plant Height, PBL (cm): primary branch length, LBB(cm): length of branch bearing, NNPB(No): number of nodes on primary branch, PBINL(cm): primary branch internode length, LL(cm): leaflet length, PtL(cm): petiole length, LW (cm): leaf width, and ODAP: oxalyl diamino propionic acid.

Figure 1. The mean seed ODAP contents of 25 grass pea accessions were evaluated at EBSU, Abakaliki, Nigeria, in 2019.

Table 3. Estimates of genetic parameters for 13 traits and ODAP among 25 accessions of grass pea evaluated at EBSU, CAS campus (2019).

			^Genetic parameters
^Characters	^Range	^Average	^(σ2e)	^(σ2g)	^(σ2p)	^GCV(%)	^PCV(%)	^H2%	^GA	^GAM%
^DE	^8-12	^9.54	^0.28	^0.55	^0.84	^7.80	^9.60	^66.00	^1.24	^13.02
^DF	^58-99.5	^80.16	^2.59	^109.16	^111.75	^13.03	^13.19	^97.68	^21.23	^26.49
^NPB	^2.6-6.8	^3.96	^0.18	^0.47	^0.64	^17.28	^20.26	^72.80	^1.20	^30.32
^NSB	^5.6-34.4	^16.82	^0.5	^47.40	^47.89	^40.93	^41.14	^98.96	^14.08	^83.71
^NBPP	^8.8-38.6	^20.77	^0.47	^50.69	^51.16	^34.28	^34.44	^99.09	^14.57	^70.16
^PH	^47.34-90.2	^68.68	^6.51	^55.74	^62.25	^10.87	^11.49	^89.54	^14.53	^21.15
^PBL	^46.1-83.3	^66.71	^6.48	^54.70	^61.18	^11.09	^11.73	^89.42	^14.38	^21.55
^LBB	^1-4.02	^1.96	^0.02	^0.21	^0.23	^23.47	^24.56	^91.37	^0.90	^46.13
^NNPB	^18.4-32.6	^27.19	^1.05	^7.16	^8.21	^9.84	^10.54	^87.21	^5.14	^18.89
^PBINL	^2.13-3.03	^2.53	^0.005	^0.04	^0.04	^7.74	^8.23	^88.46	^0.38	^14.96
^LL	^3.08-4.25	^3.65	^0.01	^0.03	^0.04	^4.88	^5.59	^76.00	^0.32	^8.74
^LW	^0.43-0.66	^0.52	^0.0002	^0.002	^0.002	^8.67	^9.09	^91.05	^0.09	^17.01
^ODAP	^0.16-0.9	^0.44	^0.0002	^0.021	^0.021	^32.82	^32.94	^99.29	^0.30	^67.23

Keywords: Ch, character; σ²g, genotypic variance; (σ ²p): phenotypic variance; GCV, genotypic coefficient of variation; PCV, phenotypic coefficient of variation; H², broad sense of heritability; DE, days to emergence; DF, days to flowering; NPB (No.): number of primary branches, NSB(No): number of secondary branches, NBPP(No): number of branches per plant, PH(cm): plant height, PBL(cm): primary branch length, LBB (cm): length of branch bearing, NNPB(No): number of nodes on the primary branch, PBINL(cm): primary branch internode length, LL(cm): leaflet length, LW (cm): leaf width, ODAP: oxalyl di amino propionic acid.

Data availability statement

The data backing up this finding can be obtained through the corresponding author, who will provide it upon reasonable request.