Detection of bla_IMP4 and bla_NDM1 harboring Klebsiella pneumoniae isolates in a university hospital in Malaysia

Figures & data

Table 1 Antibiotic susceptibility patterns of Carbapenem-resistant Klebsiella pneumoniae (CRKP)

					Antibiotic tested and zone of inhibition (mm)
Isolate	ETP	MEM	IPM	SXT	AK	CN	NET	TZP	AMC	CIP	FEP	CTX	CAZ	CXM
1	15	14	19	6	22	20	21	24	12	19	13	6	6	6
2	14	15	18	6	20	18	17	19	11	16	10	6	6	6
3	7	11	10	6	13	6	8	6	6	10	6	6	6	6
4	15	15	19	6	23	19	17	22	11	19	10	6	6	6
5	14	14	15	6	19	17	15	21	11	14	8	6	6	6
6	17	14	18	6	21	15	19	25	14	20	16	9	6	6
7	11	13	15	6	17	6	6	6	9	6	9	6	6	6
8	14	15	18	26	6	6	6	6	10	6	11	6	6	6
9	7	7	7	6	6	6	6	6	6	6	6	6	6	6
10	12	14	17	6	6	6	6	6	8	6	9	6	6	6
11	10	12	14	6	6	10	6	6	6	9	6	6	6	6
12	12	16	17	6	6	6	6	6	6	6	6	6	6	6
13	14	14	17	6	6	6	6	6	6	6	10	6	6	6

ETP, ertapenem; IMP, imipenem; MEM, meropenem; SXT, trimethoprim–sulfamethoxazole; AK, amikacin; CN, gentamicin; NET, netilmycin; TZP, piperacillin–tazobactam; AMC, amoxicillin–clavulanate; CIP, ciprofloxacin; FEP, cefepime; CTX, cefotaxime; CAZ, ceftazidime; CXM, cefuroxime.

Table 2 List of GeneBank accession numbers for bla _NDM1 and bla _IMP4 reference

Beta lactamase genes	GenBank Accession No.
bla NDM1	KC539432.1; KC539430.1; KC310727.1; JF798499.1; KF016990.1; AP012055.1; JN157804.1
bla IMP4	JX517203.1; KF250428.1; KF184388.1; JX457479.1; JN106667.1; AJ609296.3; FJ384365.1

Table 3 Summary of phenotypic and genotypic characteristics of carbapenem-resistant K. pneumoniae

	MIC (µg/ml) of carbapenem
Isolate	IPM	MEM	MHT	CD-APB	CD-EDTA	Gene detected
1	0.5	2	+	–	+	bla IMP4
2	4	>32	+	–	+	bla IMP4
3	>32	>32	+	–	+	bla IMP4
4	8	>32	+	–	+	bla IMP4
5	4	>32	+	–	+	bla IMP4
6	0.25	2	+	–	+	bla IMP4
7	>32	>32	+	–	+	bla NDM1
8	>32	>32	+	–	+	bla NDM1
9	>32	>32	+	–	+	bla NDM1
10	16	8	+	–	+	bla NDM1
11	>32	>32	+	–	+	bla NDM1
12	16	8	+	–	+	bla NDM1
13	>32	>32	+	–	+	bla NDM1

MIC, minimal inhibitory concentration; IPM, imipenem; MEM, meropenem; MHT, modified Hodge test; CD-APB, combined disc with 3′ aminophenylboronic acid; CD-EDTA, combined disc with ethylene-diaminetetra-acetic acid.