Comparison of the ForenSeq Signature Prep and ForenSeq MainstAY kits to traditional capillary electrophoresis kits for STR profile analysis from various DNA sources

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ABSTRACT

Comparisons of DNA profiles recovered from the crime-scene and a person through short tandem repeat (STR) analysis using capillary electrophoresis (CE) is the gold standard method of determining a match. A comparison of STR profiles generated using CE to massively parallel sequencing (MPS) methods was undertaken. The results showed CE methods recovered high percentages of autosomal or Y-STR markers individually, however, MPS produced more information at low inputs and a higher number of STR markers overall, showing the potential of MPS to provide additional data to assist in solving major crimes.

KEYWORDS:

• Massively parallel sequencing
• capillary electrophoresis
• short tandem repeats
• forensic DNA

Acknowledgments

Thank you to QIAGEN for providing advice and troubleshooting through this trial.

Disclosure statement

No potential conflict of interest was reported by the author(s).