Characterization of a Hyaluronate Lyase from Variovorax sp. J25

ABSTRACT

Oligosaccharides produced by degradation of hyaluronan (HA) can be applied in functional foods. In this study, Variovorax sp. J25 with high secreted HA lyase activity (16.0 U/mL), was isolated from soil, and its properties were investigated. The activities toward chondroitin sulfates A and C (CS-A and CS-C) were much lower, at 0.439 and 0.325 U/mL, respectively. The molecular weight of the purified HA lyase was around 73 kDa. The optimal reaction pH and temperature were approximately 6.7 and 50°C, respectively. The amounts of sugar produced from HA, CS-A, and CS-C were 550, 175, and 271 mg/g after reaction for 24 h, respectively. The degradation of CS-A and CS-C proceeded gradually. The major product from HA was disaccharide [ΔUA–GlcNAc], and tetrasaccharide [(ΔUA–GlcNAc)₂] was also detected (GlcNAc: N-acetylglucosamine, ΔUA: 4-deoxy-α-L-threo-hex-4-enepyranosyluronic acid). In the degradation of CS-A and CS-C, disaccharide [ΔUA–GalNAc-OSO₃H] was released (GalNAc: N-acetylgalactosamine).

KEYWORDS:

• Hyaluronate lyase
• chondroitin sulfate
• hyaluronan oligosaccharide
• N-acetylglucosamine
• 4-deoxy-α-L-threo-hex-4-enepyranosyluronic acid

Acknowledgments

We thank Edanz (https://jp.edanz.com/ac) for editing a draft of this manuscript.

Disclosure statement

No potential conflict of interest was reported by the author(s).