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Research Article

Synergistic activity of Thymus capitatus essential oil and cefotaxime against ESBL-producing Klebsiella pneumoniae

Walid Ben Selmaa Department of Biochemistry, Faculty of Medicine, Laboratory of biological and genetic markers studying for early diagnosis and follow-up of neurological diseases (LR18ES47), Sousse, Tunisia;b Higher Institute of Applied Sciences and Technology, Mahdia, TunisiaCorrespondence[email protected]
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Sana Alibic Research Unit Analysis and Process Applied to the Environment UR17ES32, Higher Institute of Applied Sciences and Technology, Mahdia, TunisiaView further author information
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Mohamed Ferjenia Department of Biochemistry, Faculty of Medicine, Laboratory of biological and genetic markers studying for early diagnosis and follow-up of neurological diseases (LR18ES47), Sousse, TunisiaView further author information
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Samira Ghezald Laboratory of Microbiology, Farhat Hached University Hospital, Sousse, TunisiaView further author information
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Najla Gallalad Laboratory of Microbiology, Farhat Hached University Hospital, Sousse, TunisiaView further author information
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Amir Belghouthid Laboratory of Microbiology, Farhat Hached University Hospital, Sousse, TunisiaView further author information
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Ali Gargourie Biotechnology center of Sfax, Laboratory of Molecular biology, Sfax, TunisiaView further author information
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Manel Marzouka Department of Biochemistry, Faculty of Medicine, Laboratory of biological and genetic markers studying for early diagnosis and follow-up of neurological diseases (LR18ES47), Sousse, Tunisia;d Laboratory of Microbiology, Farhat Hached University Hospital, Sousse, Tunisia;f Department of Microbiology, Faculty of medicine, University of Sousse, Sousse, TunisiaView further author information
&
Jalel Boukadidaa Department of Biochemistry, Faculty of Medicine, Laboratory of biological and genetic markers studying for early diagnosis and follow-up of neurological diseases (LR18ES47), Sousse, Tunisia;d Laboratory of Microbiology, Farhat Hached University Hospital, Sousse, Tunisia;f Department of Microbiology, Faculty of medicine, University of Sousse, Sousse, TunisiaView further author information
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Received 06 Aug 2023, Accepted 02 Nov 2023, Published online: 12 Nov 2023
 
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ABSTRACT

The objective of the current study was to evaluate the interaction between Tunisian Thymus capitatus essential oil (EO) and cefotaxime against Extended-Spectrum Beta-lactamases (ESBLs) producing Klebsiella pneumoniae hospital strains. GC-MS revealed that the major component of EO was found to be carvacrol (69.28%). The EO exerts an advanced bactericidal effect against all strains. Synergy between EO and cefotaxime was obtained by combined disk diffusion and checkerboard techniques. Combined use of EO and cefotaxime reduced the MIC of imipenem by 8- to 128-fold for all strains (fractional inhibitory concentration index ˂ 0.5, synergy). The time kill curve assay confirmed the advanced activity of combinatory effects of EO and cefotaxime, with total reduce of bacterial number (CFU/mL) after 6 h of culture. Synergistic activity of the combination between EO and cefotaxime constitute an important strategy as therapeutical option to combat infections caused by ESBLs producing Klebsiella pneumoniae.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

Financial support was provided by the Ministry of Higher Education, Scientific Research, and Technology (LR18ES47) Tunisia.Informed Consent Statement:Not applicable.

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