The gut microbial signatures of patients with lacunar cerebral infarction

ABSTRACT

Background

Emerging evidence revealed that gut microbial dysbiosis is involved in the pathogenesis of multiple neurological diseases, but there is little available data on the relationship between gut microbiota and lacunar cerebral infarction (LCI).

Methods

Fecal samples from acute LCI patients (n = 65) and matched healthy controls (n = 65) were collected. The compositions and potential functions of the gut microbiota were estimated.

Results

The results showed that there were significant gut microbial differences between LCI and control groups. Patients with LCI had higher abundances of genus Lactobacillus, Streptococcus, Veillonella, Acidaminococcus, Bacillus, Peptoclostridium, Intestinibacter, Alloscardovia and Cloacibacillus but lower proportions of genus Agathobacter and Lachnospiraceae_UCG-004. Investigating further these microbes such as Lactobacillus and Veillonella were correlated with clinical signs. Moreover, we found that 9 gene functions of gut microbiota were different between LCI patients and controls, which were associated with amino acid metabolism and inflammatory signal transduction. Notably, four optimal microbial markers were determined, and the combination of Streptococcus, Lactobacillus, Agathobacter, Lachnospiraceae_UCG-004 and the three risk factors achieved an area under the curve (AUC) value of 0.854 to distinguish LCI from controls.

Conclusion

These findings revealed the characterizing of gut microbiota in LCI patients and provided potential microbial biomarkers for clinical diagnosis of LCI.

KEYWORDS:

• Lacunar cerebral infarction
• gut microbiota
• microbial biomarkers
• prediction
• stroke
• 16s rRNA sequencing
• KEGG pathways
• inflammation

Disclosure statement

No potential conflict of interest was reported by the author(s).

Author contributions

Conception or design of the work: J.S., Q. T.; Data collection and analysis: J.M., H. X., C. Y., J. S., J. C., Q. C; Drafting the article: J.M.

Data availability statement

The datasets used during the current study are available from the corresponding author on reasonable request.

Additional information

Funding

This work was supported by Clinical Medical Research Project of Zhejiang Medical Association [grant number 2022ZYC-D10].

Notes on contributors

Jiaying Ma

Jiaying Ma. Publication: Chen J, Chi B, Ma J, Zhang J, Gu Q, Xie H, Kong Y, Yao S, Liu J, Sun J, Chen S. Gut microbiota signature as predictors of adverse outcomes after acute ischemic stroke in patients with hyperlipidemia. Front Cell Infect Microbiol. 2022 Nov 24; 12:1073113.

Huijia Xie

Huijia Xie. Publication: 1. Yao S, Xie H, Wang Y, Shen N, Chen Q, Zhao Y, Gu Q, Zhang J, Liu J, Sun J, Tong Q. Predictive microbial feature analysis in patients with depression after acute ischemic stroke. Front Aging Neurosci. 2023 Mar 23; 15:1116065. 2. Chen J, Chi B, Ma J, Zhang J, Gu Q, Xie H, Kong Y, Yao S, Liu J, Sun J, Chen S. Gut microbiota signature as predictors of adverse outcomes after acute ischemic stroke in patients with hyperlipidemia. Front Cell Infect Microbiol. 2022 Nov 24; 12:1073113.

Chengxiang Yuan

Chengxiang Yuan. Publication: 1. Yuan CX, Zhang YN, Chen XY, Hu BL. Association between malnutrition risk and hemorrhagic transformation in patients with acute ischemic stroke. Front Nutr. 2022 Oct 5; 9:993407. 2. Zhu BL, Hu AY, Huang GQ, Qiu HH, Hong XC, Hu PL, Yuan CX, Ruan YT, Yang B, He JC. Association Between Obesity and Post-stroke Anxiety in Patients With Acute Ischemic Stroke. Front Nutr. 2021 Nov 25; 8:749958.

Jie Shen

Jie Shen. Publication: 1. Cao YG, Li Y, Shen J, Shao MM, Lin XD, Zhu JJ, Huang XY, Cheng ZC, Yan JY, Chen KY. Clinical activity changes in the neurology department of Wenzhou during the COVID-19 pandemic: an observational analysis. Neurol Sci. 2021 May;42(5):1653-1659. 2. Shen J, Tao Z, Chen W, Sun J, Li Y, Fu F. Malignant Isolated Cortical Vein Thrombosis as the Initial Manifestation of Primary Antiphospholipid Syndrome: Lessons on Diagnosis and Management From a Case Report. Front Immunol. 2022 Apr 25; 13:882032.

Jiaxin Chen

Jiaxin Chen. Publication: Chen J, Chi B, Ma J, Zhang J, Gu Q, Xie H, Kong Y, Yao S, Liu J, Sun J, Chen S. Gut microbiota signature as predictors of adverse outcomes after acute ischemic stroke in patients with hyperlipidemia. Front Cell Infect Microbiol. 2022 Nov 24; 12:1073113.

Qionglei Chen

Qionglei Chen. Publication: Yao S, Xie H, Wang Y, Shen N, Chen Q, Zhao Y, Gu Q, Zhang J, Liu J, Sun J, Tong Q. Predictive microbial feature analysis in patients with depression after acute ischemic stroke. Front Aging Neurosci. 2023 Mar 23; 15:1116065.

Jiaming Liu

Jiaming Liu. is a professor. Publication: 1. Sun J, Zhang Y, Kong Y, Ye T, Yu Q, Kumaran Satyanarayanan S, Su KP, Liu J. Microbiota-derived metabolite Indoles induced aryl hydrocarbon receptor activation and inhibited neuroinflammation in APP/PS1 mice. Brain, Behavior, and Immunity 2022;106:76-88. 2. Sun J, Li H, Jin Y, Yu J, Mao S, Su KP, Ling Z, Liu J. Probiotic Clostridium butyricum ameliorated motor deficits in a mouse model of Parkinson's disease via gut microbiota-GLP-1 pathway. Brain, Behavior, and Immunity 2021;91:703-715.

Qiuling Tong

Qiuling Tong is an associate chief physician. Publication: 1. Yao S, Xie H, Wang Y, Shen N, Chen Q, Zhao Y, Gu Q, Zhang J, Liu J, Sun J, Tong Q. Predictive microbial feature analysis in patients with depression after acute ischemic stroke. Front Aging Neurosci. 2023 Mar 23; 15:1116065. 2. Li J, Chen W, Yi Y, Tong Q. miR-219-5p inhibits tau phosphorylation by targeting TTBK1 and GSK-3β in Alzheimer's disease. J Cell Biochem. 2019 Jun;120(6):9936-9946.

Jing Sun

Jing Sun is a professor. Publication: 1. Yao, S., Xie, H., Wang, Y., Shen, N., Chen, Q., Zhao, Y., Gu, Q., Zhang, J., Liu, J., Sun J., Tong, Q.: Predictive microbial feature analysis in patients with depression after acute ischemic stroke. Frontiers in Aging Neuroscience 2023, 15:1116065. 2. Sun J, Xu J, Ling Y, Wang F, Gong T, Yang C, Ye S, Ye K, Wei D, Song Z, Chen D, Liu J. Fecal microbiota transplantation alleviated Alzheimer's disease-like pathogenesis in APP/PS1 transgenic mice. Translational Psychiatry 2019; 9:189. 3. Ling Y, Gu Q, Zhang J, Gong T, Weng X, Liu J, Sun J. Structural change of gut microbiota in patients with post-stroke comorbid cognitive impairment and depression and its correlation with clinical features. Journal of Alzheimer's Disease 2020; 77:1595-1608.