Application and potential of multifrequency ultrasound in juice industry: Comprehensive analysis of inactivation and germination of Alicyclobacillus acidoterrestris spores

Abstract

The majority of acidic fruits are perishable owing to their high-water activity, which promotes microbial activity, thus exhibiting metabolic functions that cause spoilage. Along with sanitary practices, several treatments are used during processing and/or storage to inhibit the development of undesirable bacteria. To overcome the challenges caused by mild heat treatment, juice manufacturers have recently increased their involvement in developing novel non-thermal processing procedures. Ultrasonication alone or in combination with other hurdle technologies may be used to pasteurize processed fruit juices. Multifrequency ultrasound has gained popularity due to the fact that mono-frequency ultrasound has less impact on bacterial inactivation and bioactive compound enhancement of fruit juice. Here, we present and discuss the fundamental information and technological knowledge of how spoilage bacteria, specifically Alicyclobacillus acidoterrestris, assemble resistant spores and inactivate and germinate dormant spores in response to nutrient germinants and physical treatments such as heat and ultrasound. To the authors’ knowledge, no prior review of ultrasonic inactivation and germination of A. acidoterrestris in fruit juice exists. Therefore, this article aims to provide a review of previously published research on the inactivation and germination of A. acidoterrestris in fruit juice by ultrasound and heat.

Keywords:

• Sublethal injuries
• Alicyclobacillus acidoterrestris
• dipicolinic acid release
• thermosonication
• ultrasound mechanisms
• spores

Credit authorship contribution statement

Hafida Wahia: Supervision, validation, conceptualization, writing original draft, review and editing; Cunshan Zhou: Supervision, validation, project administration, funding acquisition; Olugbenga Abiola Fakayode: Supervision, reviewing and editing; Abdullateef Taiye Mustapha: Reviewing and editing Mokhtar Dabbour: Reviewing and editing.

Disclosure statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Additional information

Funding

The authors are grateful for the support provided by Jiangsu Funding Program for Excellent Postdoctoral Talent (2022ZB674), the National Key Research and Development Program of China (2017YFD0400903-01, 2016YFD0400705-04), Science and Technology Major Project of Anhui (18030701152).