Assessing the anti-collagenase activity and phytochemical evaluation of eight plants cultivated in Egypt; developing a validation method for the ellagic acid content in Chrysophyllum oliviforme using HPLC-DAD

Abstract

Preventing aging signs is a vast cosmetic quest hence; the authors felt the necessity to focus on emerging new plant extracts by the evaluation of anti-aging potential of eight plants cultivated in Egypt. Total phenolics (TPC), total flavonoids content (TFC) and collagenase assay were performed; only four plants were subjected to (ORAC) assay, ferrozine iron metal chelation assay and (HPLC) analysis against polyphenolic standard; validation method according to ICH guidelines for ellagic acid content in C. oliviforme by HPLC-DAD was performed, molecular docking simulation was implemented by (MOE) module. C. oliviforme exhibited the highest anti-collagenase with the lowest (IC₅₀), (TPC = 299.70 ± 16.97 mg/GAE), method of validation of C. oliviforme extract following the ICH guidelines of ellagic acid content (147.446 ± 0.00041 mg/g); C. oliviforme was the most potent extract and was standardized to be reproducible for the industrial scale.

Graphical Abstract

Keywords:

• C. oliviforme
• ellagic acid
• HP LC standardization
• MOE module
• MMP-1
• Anti-collagenase assay

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The author(s) reported there is no funding associated with the work featured in this article.