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Original Articles

Genetic Variation in Tall Fescue (Festuca arundinacea Schreb) Populations Based on Seed Storage Protein Polymorphism

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Pages 390-398 | Published online: 03 Dec 2010
 

Abstract

Tall fescue (Festuca arundinacea Schreb) is one of the main perennial forage grasses that naturally grows in temperate pasture and rangelands in northern and western Iran. The objective of this study was to determine the genetic variability among 15 populations of tall fescue via SDS-PAGE analysis of seed storage proteins. This technique generated a total of 46 protein patterns that showed two types of polymorphism: presence or absence of patterns and their intensities. Populations G15 and G24 with average values of 41 and 28 had higher and lower band numbers, respectively. Cluster analysis using UPGMA method was performed on resultant data obtained from SDS-PAGE for seed proteins. Three distinct groups were identified from the cluster analysis at the similarity coefficient level of 0.75. The bands of 65 and 70 kDa can be used as protein markers to identify populations. We concluded that seed storage protein polymorphism could be a useful marker in population identification, study of genetic diversity and improving the efficiency of grass breeding programs.

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