Development and Validation of Multiplex Quantitative PCR Assay for Detection of Helicobacter pylori and Mutations Conferring Resistance to Clarithromycin and Levofloxacin in Gastric Biopsy [Corrigendum]

This article refers to:

Development and Validation of Multiplex Quantitative PCR Assay for Detection of Helicobacter pylori and Mutations Conferring Resistance to Clarithromycin and Levofloxacin in Gastric Biopsy

Binmaeil H, Hanafiah A, Mohamed Rose I, Raja Ali RA. Infect Drug Resist. 2021;14:4129–4145.

The authors have advised that Table 1 on page 4132 is incorrect. There is an error in the clarithromycin resistant gene reverse primer and UreA probe sequences.

The authors apologize for these errors. The correct Table 1 is as follows.

Table 1 Oligonucleotides Sequence Used in This Study

Primers/Probes	Sequences (5′→3′)	Targets	Product (bp)
UreA-F^a	AGTTCCTGGTGAGTTGTTCTT	ureA	120
UreA-R^b	TGGAAGTGTGAGCCGATTT
Cla-F^a	CTGCGCATGATATTCCCGTT	23S rDNA	157
Cla-R^b	CGACCTGCATGAATGGCGTA
Lev-F^a	CGCCATCAATAGAGCCAAAG	gyrA	136
Lev-R^b	GTGGGTGATGTGATTGGTAAAT
UreA-P^c	FAM-AAATGTTGGCGACAGACCGGTTC-TAMRA	ureA
Cla-PWT^c	Cy5-AAGGTCCACGGGGTCTTTCCGTC-BHQ2	Wild type
Cla-P2142G^c	HEX-AGGTCCACGGGGTCTTCCCGTCT-BHQ2	A2142G
Cla-P2143G^c	FAM-AGGTCCACGGGGTCTCTCCGTCTT-BHQ2	A2143G
Lev-N87WT^c	HEX-CCG+C+G+TTA+T+CG-IowaBlack^®FQ	Wild type
Lev-N87K^c	6-FAM-CCG+C+T+TT+A+T+CGC-IowaBlack^®FQ	N87K
Lev-N87I^c	Cy5-CCG+C+A+AT+A+T+CGC-IowaBlack^®RQ-Sp	N87I
Lev-D91WT^c	HEX-TA+T+G+AT+GCG+CT-IowaBlack^®FQ	Wild type
Lev-D91N^c	Cy5-TTA+T+A+ATG+C+G+CTAGT-IowaBlack^®RQ-Sp	D91N
Lev-D91Y^c	6-FAM-TTA+T+T+ATG+C+G+CTAGT-IowaBlack^®FQ	D91Y

Note: ^aForward primer, ^bReverse primer, ^cTaqMan probe.